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Phosphorus is a major essential macronutrient for plant growth, and most of the phosphorus in soil remains in insoluble form. Highly efficient phosphate-solubilizing bacteria can be used to increase phosphorus in the plant rhizosphere. In this study, 13 isolates were obtained from waste mushroom residues, which were composed of cotton seed hulls, corn cob, biogas residues, and wood flour. NBRIP solid medium was used for isolation according to the dissolved phosphorus halo. Eight isolates produced indole acetic acid (61.5%), and six isolates produced siderophores (46.2%). Three highest phosphate-dissolving bacterial isolates, namely, M01, M04, and M11, were evaluated for their beneficial effects on the early growth of tomato plants (Solanum lycopersicum L. Wanza 15). Strains M01, M04, and M11 significantly increased the shoot dry weight by 30.5%, 32.6%, and 26.2%, and root dry weight by 27.1%, 33.1%, and 25.6%, respectively. Based on 16S rRNA gene sequence comparisons and phylogenetic positions, strains M01 and M04 belonged to the genus Acinetobacter, and strain M11 belonged to the genus Ochrobactrum. The findings suggest that waste mushroom residues are a potential resource of plant growth-promoting bacteria exhibiting satisfactory phosphate-solubilizing for sustainable agriculture.
The first description is presented of nucleotide sequence of exon 2 of caprine melatonin receptor 1b (MT2). No polymorphisms of MT2 gene were detected between high fertility and year-round estrous goat breeds and low fertility and seasonal estrous goat breeds. It is likely that exon 2 of MT2 gene is not associated with fertility or reproductive seasonality in goat breeds. The nucleotide sequence of exon 2 of MT2 gene of Jining Grey goats shows much closer phylogenetic relation to the MT2 of sheep (97%) and cattle (94%) than to that of pig (84%), human (80%) and mouse (74%). A rather high nucleotide identity (62-64%) with the melatonin receptor 1a (MT1) of goat, sheep, human and mouse was also found. The caprine MT2 contains the same NAXXY motif in transmembrane 7 as the other melatonin receptors. Both DRY and CYVCR motifs were detected just downstream from its third transmembrane domain (the same as in sheep and cattle) rather than NRY and CYICH found in other melatonin receptor groups.
The ammonium sulfate ((NH₄)₂SO₄) seed aerosols commonly found in the Chinese urban atmosphere could affect the formation and aging of secondary organic aerosols (SOA). Aging of aromatic SOA is performed using UV-irradiation of 1,3,5-trimethylbenzene (135-TMB)/CH₃ONO/NO/air mixtures with high concentrations (~100 μg/m³) of (NH₄)₂SO₄ seeds in the laboratory chamber in this study. The particulate products of SOA were measured using an aerosol laser time-of-flight mass spectrometer (ALTOFMS) in real-time, and fuzzy c-means (FCM) was applied to the mass spectra organic species for clusters. Experimental results indicated that methylglyxoal, 2-methyl-4-oxo-2-pentenal, 4-methyl-1H-imidazole, 4-methyl-imidazole-2-acetaldehyde, and other imidazole derivative compounds are the principal products in the aged particles. Imidazole compounds that can absorb solar radiation effectively were newly detected in the aged 135-TMB SOA with high concentrations of (NH₄)₂SO₄ seed aerosols. These would provide new information for discussing the 135-TMB SOA aging mechanism.
The inhibin βB (INHBB) gene was studied as a candidate gene for the prolificacy of Jining Grey goats. According to the sequence of bovine INHBB gene, two pairs of primers were designed to detect single nucleotide polymorphism in exon 2 of INHBB gene in high-prolificacy (Jining Grey)and low-prolificacy (Inner Mongolia Cashmere and Angora) goats by polymerase chain reactionsingle strand conformation polymorphism method. Only the amplified products of primer P2 showed polymorphism. Three genotypes (AA, AB and BB) were identified in Jining Grey and two (AB and BB) in Inner Mongolia Cashmere and Angora goats. Sequencing revealed one single nucleotide mutation (A→G) at base 782 of exon 2 of INHBB gene in BB genotype compared to genotype AA.
The Arabidopsis Ethylene-Insensitive 2 (EIN2) gene was shown to be involved in the regulation of abiotic and biotic stresses, such as ozone stress, high salt, oxidative stress, lead and disease resistances. However, it is unclear whether EIN2 plays a role in mediating the ultraviolet (UV)-B response in Arabidopsis. Here, we show that EIN2 is involved in the regulation of the UV-B response. The EIN2 expression was repressed by UV-B exposure, and ein2-1 mutant plants were more tolerant to UV-B than wild-type plants, as indicated by analysis of survival rates. Significant higher increases in flavonoids and anthocyanins were detected in ein2-1 plants than in wild-type plants treated with or without UV-B treatment, which was associated, at least in part, with constitutively increased transcript levels of two key genes CHALCONE SYNTHASE (CHS) and CINNAMATE 4-HYDROXYLASE (C4H) involved in the biosynthesis pathway of flavonoid and anthocyanins. These results suggest that EIN2 mediates the UV-B response, at least in part, via the modulation of expression of CHS and C4H genes.
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