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1

Zmiany w metabolizmie glikozaminoglikanów pod wpływem działania ołowiu

100%
Turczyn B., Skoczynska A., Wojakowska A.
Kosmetologia Estetyczna
|
2015
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tom 04
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nr 2
2

Metabolic response of narrow leaf lupine (Lupinus angustifolius ) plants to elicitation and infection with Colletotrichum lupini under field conditions

88%
Wojakowska A., Kulak K., Jasinski M., Kachlicki P., Stobiecki M.
Acta Physiologiae Plantarum
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2015
|
tom 37
|
nr 08
Lupine (Lupinus spp.) is one of the crop plants from the Fabaceae family cultivated on a moderate scale in Europe, Australia and South America; however, its cultivation suffers from a severe fungal disease anthracnose caused by Colletotrichum lupini fungus. The search for resistant plant genotypes as well as methods of plant immunization against such infections is of importance. Plant interaction with pathogenic microorganisms results in complex regulation of many biochemical and physiological processes. Activation of expression of defence genes that leads to the induction of bioactive secondary metabolites biosynthesis is among them. The aim of the presented work was to investigate changes in the isoflavonoids quantities as the reaction of narrow leaf lupine (Lupinus angustifolius) plants growing in the field conditions to infection with the pathogenic fungus (C. lupini) or treatment with its phytotoxic metabolites followed after 48 h with infection with the fungus. The metabolic profiling after field experiments revealed variety-specific changes of these compounds. Elicitation of plants with fungal phytotoxin prior to infection resulted in higher levels of prenylated isoflavones, especially phytoalexins luteone and wighteone and their various glycoconjugates in comparison to those observed in plants infected only with the fungal spores. The metabolomic analyses were supported by the transcriptomic view of genes involved in isoflavonoids biosynthesis. Graphical abstract Infection of Lupinus angustifolius by Colletotrichum lupini combined with former elicitation of plants results in accumulation of prenylated isoflavonoids and change in the isoflavone prenyltransferase gene expression pattern.
3

Influence of abiotic stresses on plant proteome and metabolome changes

88%
Rodziewicz P., Swarcewicz B., Chmielewska K., Wojakowska A., Stobiecki M.
Acta Physiologiae Plantarum
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2014
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tom 36
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nr 01
Plant responses to abiotic stresses are very complex phenomena with individual characteristics for various species. Abiotic stresses (e.g. drought, salinity, flooding, cold, heat, UV radiation, heavy metals, etc.) strongly affect plant growth and development. It is estimated that they are the cause of more than 50 % of crop yield losses. Abiotic stresses are known to activate a multigene response resulting in the changes in various proteins and primary and secondary metabolite accumulation. Therefore, proteomic and metabolomic approaches are becoming very important and powerful tools used in studying plants’ reaction to various stimuli. Precise analysis of proteome and metabolome is essential for understanding the fundamentals of stress physiology and biochemistry. In this review, we focus on recent reports concerned to the influence of abiotic stresses on changes in the level of different protein groups and metabolite classes. Basic information about physicochemical methods applied to qualitative and quantitative analyses of biopolymers and natural products is also briefly presented.
4
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Lipid pattern in middle-aged inhabitants of the Lower Silesian region of Poland. The PURE Poland sub-study

64%
Skoczynska A., Wojakowska A., Turczyn B., Zatonska K., Wolyniec M., Regulska-Ilow B., Ilow R., Szuba A., Zatonski W.
Annals of Agricultural and Environmental Medicine
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2013
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tom 20
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nr 2
Introduction. A decreased serum high density lipoprotein-cholesterol (HDL-C) is a strong predictor of cardiovascular risk. However, total HDL is a very dynamic, changeable fraction, and does not perform the function of atherosclerosis markers. In the presented study, the pattern of serum lipids, including HDL-C subclasses (HDL2- and HDL3-cholesterol), in a middleaged Polish Lower Silesia population was defined. Materials and method. A group of 746 males and 1,298 females, aged 35–70, were investigated. All subjects were participants in the PURE study. Mean serum lipid levels were determined for groups selected on the basis of gender, age, cigarette smoking, drinking alcohol and place of residence (urban/rural area). The data were analyzed using STATISTICA 6.0 PL. Results. In multiple linear regression models, age was the most important independent and consistent predictor of total cholesterol (TC) and LDL cholesterol (LDL-C). The prevalence of low HDL-C (threshold 40 mg/dL in males, 50 mg/dL in females) was 16.5% for males and 22.6% for females. This gender-conditioned difference in the prevalence of low HDL-C was greater in rural (20.0% vs. 30.9%, respectively, in males and females) in comparison to urban (14.4% vs. 17.1%) areas. The lipid pattern was significantly worse in rural than in urban females. Female rural inhabitants showed higher triglycerides (TG) and lower HDL cholesterol (total and contained in subclasses HDL2 and HDL3). Simultaneously, a higher BMI, higher percent of smokers and drinkers and lower age of smoking female rural inhabitants in comparison to urban females were estimated. In the total population, cigarette smoking or drinking alcohol were associated with significant increases in TC, LDL-C and TG, also with decreased HDL-C (smoking) or HDL2-C (drinking). Two-way analysis of variance showed the existence of interaction between these risk factors in their influence on HDL-C and HDL3-C. Conclusion. In the middle-aged population of the Lower Silesian region in Poland the place of residence (urban/rural area) had a significant impact on the lipid pattern. This pattern is more atherogenic in rural women than in urban women.
5

Ionizing radiation affects protein composition of exosomes secreted in vitro from head and neck squamous cell carcinoma

64%
Jelonek K., Wojakowska A., Marczak L., Muer A., Tinhofer-Keilholz I., Lysek-Gladysinska M., Widlak P., Pietrowska M.
Acta Biochimica Polonica
|
2015
|
tom 62
|
nr 2
6
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
Content available

Drinking of chokeberry juice from the ecological farm Dzieciolowo and distensibility of brachial artery in men with mild hypercholesterolemia

64%
Poreba R., Skoczynska A., Gac P., Poreba M., Jedrychowska I., Affelska-Jercha A., Turczyn B., Wojakowska A., Oszmianski J., Andrzejak R.
Annals of Agricultural and Environmental Medicine
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2009
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tom 16
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nr 2
305-308
7

Global proteomic analysis of brain changes induced by 'lifestyle' modifications

64%
Nowacka-Chmielewska M., Liskiewicz D., Liskiewicz A., Marczak L., Wojakowska A., Przybyla M., Grabowski M., Jablonska K., Barski J. J., Malecki A.
Acta Neurobiologiae Experimentalis
|
2019
|
tom 79
|
nr Suppl.1
In the pathogenesis of central nervous system (CNS) disorders, an increasingly important role is attributed recently to unhealthy lifestyle, which consists primarily of a high caloric diet (i.e., western), chronic exposure to stress, and lack of physical activity. However, the mechanisms responsible for energy metabolism impairment induced by unhealthy lifestyles compromising CNS functions are poorly understood. Research on the effects of physical activity on the CNS is especially important, because it may result in the development of new methods of therapy inspired by natural protective mechanisms. In our study we employed a new and rarely used approach – a forced running wheel. The lack of electrical stimulus in the aforementioned system successfully makes a breakthrough in the study of animal physical activity. Physiological and behavioral responses of the organism to stress are closely related to sex. Epidemiological studies indicate that women are more vulnerable to the adverse effects of stress and despite that, most of the experimental studies are conveyed on male animals. The investigations were carried on female rats. The main goal of our study was to verify the hypothesis that regular exercise may reduce the disturbances induced by lifestyle modifications, like western diet and/or stress exposure. Adult female rats were fed with the prepared chow reproducing the human western diet and/or subjected to a stress induced by social instability. This stress protocol is characterized by a low degree of invasiveness. To evaluate if regular physical activity may reduce the adverse effects caused by diet and stress, female rats were additionally subjected to the procedure of forced physical activity. A proteomic analysis was conducted on samples obtained from the frontal cortex – a region that plays an important role in cognitive processes as well as is involved in the mechanisms engaged in the response to stress.
8

Panel of serum metabolites discriminates cancer patients and healthy participants of lung cancer screening - a pilot study

64%
Ros-Mazurczyk M., Wojakowska A., Marczak L., Polanski K., Pietrowska M., Polanska J., Dziadziuszko R., Jassem J., Rzyman W., Widlak P.
Acta Biochimica Polonica
|
2017
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tom 64
|
nr 3
9

Ionizing radiation affects profile of serum metabolites: increased level of 3-hydroxybutyric acid in serum of cancer patients treated with radiotherapy

52%
Ros-Mazurczyk M., Wojakowska A., Marczak L., Polanski K., Pietrowska M., Jelonek K., Dominczyk I., Hajduk A., Rutkowski T., Skladowski K., Widlak P.
Acta Biochimica Polonica
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2017
|
tom 64
|
nr 1
10

Anxiolytic effect of physical activity and cerebral accumulation of saturated fatty acids

52%
Liskiewicz A., Przybyla M., Wojakowska A., Marczak L., Bogus K., Nowacka-Chmielewska M., Liskiewicz D., Malecki A., Barski J. J., Lewin-Kowalik J., Toborek M.
Acta Neurobiologiae Experimentalis
|
2019
|
tom 79
|
nr Suppl.1
INTRODUCTION: The broad spectrum of the positive effects of physical activity on brain functioning is well acknowledged. Among others, it induces an improvement in mood, and a part of the rodent studies support this thesis by showing anxiolytic effects of exercise. However, the mechanism of this behavioral modification is not clear. Changes in brain metabolism may contribute to the generation of complex brain disorder phenotypes; thus, metabolomics have proven to be useful tools in studies on the central nervous system. AIM(S): The discrimination of anxiolytic level and metabolomics changes in the brain were evaluated in this study. METHOD(S): Voluntary running mice were subjected to a battery of behavioral tests (Open Field, Elevated Plus Maze, Dark/Light Box) commonly used to measure anxiety levels. Simultaneously, GC/MS analysis of hippocampal and cortical samples was performed for metabolome profiling of the running mice. RESULTS: The exercised animals showed anxiolytic behavior. Voluntary running caused an accumulation of saturated fatty acids, such as myrisitc, palmitic, heptadecanoic, and stearic acids, in the hippocampus and cortex of running mice. CONCLUSIONS: A striking observation in the present study is that a profile of saturated fatty acids that accumulates in the hippocampi and cortex of the running mice is consistent with the mixture of fatty acids that was identified as causing anxiolytic-like effects when administered to rodents.
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