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Critical factors besides treatment dose and duration need to be controlled in Pb toxicity tests in plant cell suspension cultures

100%

Sychta K., Szklarzewicz J., Slomka A., Gregoraszczuk E., Kuta E.

Acta Societatis Botanicorum Poloniae

2017

tom 86

nr 3

The study was designed to determine the proper conditions for suspension culture of Viola tricolor cells in toxicity studies of Pb at different concentrations (0, 200, 500, 1000, 2000 μM) and exposure times (24, 48, 72 h). By forming insoluble salts with ions from the medium, lead (II) nitrate added to the medium decreased the initial 5.7–5.8 pH of the medium, depending on the Pb salt concentration and light intensity. In alamarBlue assays, we found no dose- or time-dependent effect of Pb on cell viability when we did not adjust pH and did not standardize the illumination conditions to correct the effect of lead-salt-induced turbidity. When effective illumination was adjusted to correct for turbidity at the highest lead concentration and pH was adjusted to 5.7–5.8, cell viability decreased with the increase of Pb(NO₃)₂ concentration and with treatment time. These experiments demonstrate that the toxic action of lead on cells in suspension depends strongly on culture conditions, and not only on the metal concentration and duration of treatment.

Level and relationship of elements in scalp hair of males: effect of air pollution and smoking habits

86%

Grybos R., Zagrodzki P., Krosniak M., Lagan L., Szklarzewicz J., Golas J., Przybylski W.

Polish Journal of Environmental Studies

2005

tom 14

nr 1

Concentrations of Cd, Cr, Cu, Mn, Mo, Ni, Pb, Se, Tl and Zn were analyzed by ICP-MS in the scalp hair of male subjects from an urban area, three different quarters of Krakow, Poland, and from a rural area as control, which were assumed to differ in ambient pollution by metals. A questionnaire on personal data, nutritional habits, socioeconomic, occupation and health status was completed by the subjects. Significant differences (p < 0.05) were found in the levels of Cd, Cu, Mn and Ni between the locations. Using statistical methods of principal components analysis, the relationships were found between metals in the scalp hair as follows: Mn-Ni, Cr-Tl and Cd-Pb. Two separated clusters of smokers were revealed in the principal components, space suggesting other factors like environmental contamination could confound the values of parameters and relationships between them.

Ograniczanie wyników

1 Acta Societatis Botanicorum Poloniae

1 Polish Journal of Environmental Studies

2 Szklarzewicz J.

1 Golas J.

1 Gregoraszczuk E.

1 Grybos R.

1 Krosniak M.

1 Kuta E.

1 Lagan L.

1 Przybylski W.

1 Slomka A.

1 Sychta K.

1 Zagrodzki P.

1 2017

1 2005

Wyniki wyszukiwania

Critical factors besides treatment dose and duration need to be controlled in Pb toxicity tests in plant cell suspension cultures

Level and relationship of elements in scalp hair of males: effect of air pollution and smoking habits