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Ghrelin in the postnatal development of the gastrointestinal tract

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Ghrelin is a 28-amino acid peptide first isolated from rat and human stomachs. Together with the recently discovered 23-amino acid obestatin, it is derived from proghrelin by posttranslational processing. Cells immunoreactive to ghrelin are widely distributed in the gastric mucosa in domestic and laboratory animals and in humans. Ghrelin plays an important role in energy homeostasis, body weight control, and food intake, whereas obestatin seems to induce the opposite effects. Ghrelin and ghrelin receptor expression have been found in developing gastrointestinal foetal and neonatal tissues, and substantial amounts of ghrelin are present in colostrum, thereby suggesting its presumable role in perinatal development. Ghrelin was shown to positively influence weight gain, increase GH, insulin and cortisol secretion. It also stimulates gastrointestinal tissue structure and function development in weaned animals. Surprisingly, responses in suckling neonates were found to be opposite to those in weanlings. Ghrelin retarded gastric, intestinal and pancreatic development and showed a tendency to reduce body weight gain. Recent studies suggest that the biphasic effect of ghrelin in young rats on pancreas and stomach growth seems to be related to age-dependent changes of the release of anabolic IGF-1. In the perinatal period, obestatin is detected in the rat stomach, pancreas and blood plasma. The obestatin concentration in rats is abruptly reduced after birth, contrasting with an increase in the concentration of acylated ghrelin. Further progressive reduction in pancreas obestatin is observed until weaning.
Most of the strategies developed to reduce weaning diarrhea and to improve the health and performance of pigs are targeted at the post-weaning period and concern modifications of feed composition and/or supplementation with a variety of feed additives. Studies on the physiology of gastrointestinal tract development, however, clearly demonstrated a conflict between the immaturity of the gut mucosa and early weaning. A kidney bean lectin preparation (Suilectin™, Biolek, Poland) was designed to speed up the maturation of the pig gastrointestinal mucosa before weaning. The aim of the present study was to examine the effectiveness of Suilectin™ under practical conditions. The study was performed in a single farm on the total of 3575 piglets, some of which orally received a single dose of Suilectin™ when they were 10-12 d of age (3.27 ± 0.04 kg body weight, BW). Piglets were weaned when they were 25-26 d of life. Their body weight, food intake, clinical health, and veterinary costs were calculated. Suilectin™-treated pigs were characterized by a higher BW at weaning, a lower incidence of post-weaning diarrhea, lower costs of veterinary treatment, and lower mortality as compared to control.
Feeding of neonates with artificial milk formulas delays the maturation of the gastrointestinal mucosa. Na-butyrate has a complex trophic effect on the gastrointestinal epithelium in adults. The present study aimed to determine the effect of milk formula supplementation with Na-butyrate on the gut mucosa in neonatal piglets. Sixteen 3 day old piglets were randomly divided into two groups: control (C, n = 8), and Na-butyrate (B, n = 8). Animals were feed for 7 days with artificial milk formula alone (C) or supplemented with Na-butyrate (B). At the 10th day of life the piglets were sacrificed and whole thickness samples of the upper gut were taken for analyses. Administration of Na-butyrate led to significant increase in daily body weight gain as compared to control. In the duodenum, the villi length and mucosa thickness were reduced, however, in the distal jejunum and ileum, the crypt depth, villi length and mucosa thickness were increased in Na-butyrate supplemented piglets as compared to control. Supplementation with Na-butyrate did not affect the intestinal brush border enzyme activities but increased plasma pancreatic polypeptide and cholecystokinin concentrations. These results suggest that supplementation with Na-butyrate may enhance the development of jejunal and ileal mucosa in formula-fed piglets.
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