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Effects of different levels of eucalyptus oil on methane production under in vitro conditions

100%

Abdelrahman S. L., Li R. H., Elnahr M., Farouk M. H., Lou Y.

Polish Journal of Environmental Studies

2019

tom 28

nr 3

Livestock animals are seriously contributing to global warming as methane producers. Six levels (0, 2, 4, 6, 8, and 10 ml.kg⁻¹ DM) of eucalyptus oil (EuO) were investigated under in vitro conditions to study the mitigation ability for methane production, using two rations: R1 (70% forage: 30% concentrates) and R2 (60% forage: 40% concentrates). Two cannulated sheep were used as donor animals to obtain the rumen liquid. The results showed that CH₄ production levels were significantly (P≤0.05) lower in all treated groups with EuO than the control group (0 ml.kg⁻¹ DM) in both rations. The retreating for CH₄ of R1 was 32%, adding of 2 mL.kg⁻¹ DM, and was 38% in10 ml.kg⁻¹ DM. Regarding R2, the decrease ratio of methane production was 42% in 2.0 mL addition, whereas it was 46% in 10 mL of addition rate. In R2, protozoa count was significantly (P≤0.05) lower by adding the eucalyptus oil compared with the control. In conclusion, using EuO and a high-protein diet could decrease both total gas volume and methane production even with minimal oil levels (2.0 mL EuO.kg⁻¹DM). It is recommended to carry out an in vivo experiment to emphasize the effects of EuO on the ruminant.

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In vitro production of Sudanese camel (Camelus dromedarius) embryos from epididymal spermatozoa and follicular oocytes of slaughtered animals

86%

Abdelkhalek A. E., Gabr S. A., Khalil W. A., Shamiah S. H., Pan L., Qin G., Farouk M. H.

Polish Journal of Veterinary Sciences

2017

tom 20

nr 1

Application of assisted reproductive technology in camelidea, such as artificial insemination (AI) and embryo transfer, has been slow in comparison to that for other livestock species. In Egypt, there are few attempts to establish in vitro maturation (IVM) and fertilization (IVF) techniques in dromedary camel. The present study was carried out to produce Sudanese camel embryos using in vitro matured oocytes and epididymal spermatozoa. Dromedary camel ovaries were collected from abattoirs and then, the oocytes were aspirated from all the visible follicles on the ovarian surface (~2-8 mm in a diameter). Meanwhile, Fetal Dromedary Camel Serum (FDCS) was obtained from camel fetuses after slaughtering. Thereafter, only Cumulus Oocyte Complexes (COCs) were matured in vitro in the Tissue Culture Medium (TCM-199) complemented with 10% FDCS. Spermatozoa required for in vitro fertilization were collected from testes (epididymal cauda) of the slaughtered camel bulls. The results clearly showed that the maturation rate of oocytes at metaphase II was about 59.5% while the fertilization rate was around 70.4%. Intriguingly, the embryo rates determined were 13.1%, in 2-cell; 0.0%, in 4-cell; 34.7%, in 8-16% cell; 39.1%, in morula and 13.1% in a blastocyst stage. This study represented a successful in vitro production of Sudanese dromedary camel embryos from epididymal sperm cells and in vitro matured oocytes recovered from slaughtered camels.

Ograniczanie wyników

1 Polish Journal of Environmental Studies

1 Polish Journal of Veterinary Sciences

2 Farouk M. H.

1 Abdelkhalek A. E.

1 Abdelrahman S. L.

1 Elnahr M.

1 Gabr S. A.

1 Khalil W. A.

1 Li R. H.

1 Lou Y.

1 Pan L.

1 Qin G.

1 Shamiah S. H.

1 2019

1 2017

Wyniki wyszukiwania

Effects of different levels of eucalyptus oil on methane production under in vitro conditions

In vitro production of Sudanese camel (Camelus dromedarius) embryos from epididymal spermatozoa and follicular oocytes of slaughtered animals