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Anthocyanins impart red, purple and violet colour to many flowers and fruits, mainly to attract pollinators and seed dispersers, but their function and biosynthetic regulation in foliages of several plants are less studied. The red and green forma of Ocimum tenuiflorum differ in anthocyanin accumulation in leaves and provide an excellent system for exploring the course of its regulation. It was observed that red forma gradually changed to green upon transfer to a particular greenhouse with limited transmission of ultraviolet light (both UV-B and UV-A). The sequential monitoring of anthocyanin content confirmed positive correlation between visible and ultraviolet light intensity with leaf colour and antioxidant activities. An ultra-performance liquid chromatography method of < 3.5 min was developed for rapid and precise quantification of anthocyanidins. Expressions of PAL, CHS and CHI were down-regulated by low light in both forma. The F3H and F3'H genes had reduced expression in both forma and were supported by reduced levels of cyanidin in red forma plants within greenhouse. The expression of late biosynthetic genes, DFR and LDOX, also plummeted within the greenhouse. The regulatory transcription factors bHLH and WD40 were severely down-regulated within the greenhouse suggesting that bHLH and WD40 control the expression of F3'H, DFR and LDOX to regulate the biosynthesis of anthocyanin pigments in leaves of O. tenuiflorum, whereas the expression of Myb remained almost unaffected.
Biological control of plant diseases is strongly emerging as an effective alternative to the use of chemical pesticides and fungicides. Stress tolerance is an important attribute in the selection of bacteria for the development of microbial inoculants. Fourteen salt-tolerant bacteria showing different morphological features isolated from the rhizosphere of maize were evaluated for different plant growth-promoting activities. All isolates showed auxin production ranging from 5 to 24 μg ⋅ ml–1 after 48 h incubation in tryptophan supplemented media. Phosphate solubilization ranged from 15 to 419 μg ⋅ ml–1. 1-aminocycloproprane- 1-carboxylate (ACC) deaminase activity was shown by 6 isolates, ammonia production by 9 isolates, siderophore production by 8 isolates while HCN production by 4 isolates. Four bacterial isolates with all plant growth-promoting properties also showed strong antagonistic activities against Fusarium oxysporum, F. verticillioides, Curvularia lunata and Alternaria alternata and abiotic stress tolerance against salinity, temperature, pH and calcium salts. Two selected bacterial isolates significantly enhanced the growth of pea and maize test plants under greenhouse conditions. The bacterial isolate M1B2, which showed the highest growth promotion of test plants, was identified as Bacillus sp. based on phenotypic and 16S rDNA gene sequencing. The results indicated that Bacillus sp. M1B2 is a potential candidate for the development of microbial inoculants in stressful environments.
Methanol and ethanol extract showed antibacterial activity in range of 0-11 at 10 mg/mL at against all tested bacterial species. Methanol extract with amoxicillin exhibited effective inhibition E. coli. This combination showed antagonistic effect against B. subtilis and S. aureus. Interaction of ethanol extract with amoxicillin also showed effective inhibition against E. coli and decreased activity against B. subtilis and S. aureus. Methanol extract did not show inhibition against P. aeruginosa but it enhanced activity of ciprofloxacin on combination. Ethanol extract also exhibited effective inhibition against P. aeruginosa on combination with ciprofloxacin. But antagonist effect was observed on interaction of both extract with ciprofloxacin against other bacterial species. Interaction of methanol and ceftazidime showed mostly decreased or indifferent effect against all bacterial species. Ethanol extract with ceftazidime resulted in higher inhibition against E. coli. Combination of methanol extract with erythromycin showed higher inhibition against E. coli and also very effective inhibition against S. aureus. This combination resulted in decreased activity against B. subtilis. Ethanol extract with erythromycin also showed synergistic effect against S. aureus and effective inhibition against E. coli. These results indicate that both methanol and ethanol extract has potential to enhance the combination effect with erythromycin against S. aureus and E. coli.
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