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1

Knockdown of Sucla2 decreases the viability of mouse spermatocytes by inducing apoptosis through injury of the mitochondrial function of cells

100%
Huang S., Wang J., Wang L.
Folia Histochemica et Cytobiologica
|
2016
|
tom 54
|
nr 3
2
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
Content available

Helminth parasites of the Nile Rat, Arvicanthis niloticus, from Shendi area, Sudan

88%
Sulieman Y., El-Tayeb R. E., Srimek N., Huang S., Pengsakul T.
Annals of Parasitology
|
2019
|
tom 65
|
nr 3
Eighty nine rats, Arvicanthis niloticus, were collected from the horticultural fields of Shendi area in Sudan, between January and June 2018, and examined for the first time for helminth parasites. Thirty seven (41.6%) of the collected rats were infected, with an overall mean intensity of 4.4 helminths per a rat. A total of 6 helminth species were identified including three nematodes (Nippostrongylus brasiliensis, Monanema nilotica and Capillaria hepatica) and three cestodes (Hymenolepis diminuta, H. nana and Taenia taeniae formis). The most prevalent helminth was found to be the nematode, N. brasiliensis (21.3%), followed by the cestode, H. diminuta (10.1%), while the least was the nematode, C. hepatica (1.1%). Higher prevalence and intensity of infection were observed among older rats. Likewise, male rats were found to harbor a higher prevalence and intensity of infection. In conclusion, the rat, A. niloticus in Shendi area has found to be parasitized by various species of helminths, which some are of zoonotic importance, thus, any possible contact between this rat and humans or their pets may pose potential risk to public health.
3

Distribution and risk assessment of heavy metals in soils from a typical Pb-Zn mining area

76%
Huang S., Yuan C., Li Q., Yang Y., Tang C., Oyuang K., Wang B.
Polish Journal of Environmental Studies
|
2017
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tom 26
|
nr 3
This study focuses on soil heavy metal pollution and the potential ecological risks associated with mining activities. For this work we collected 83 topsoil samples and 11 soil profile samples from a typical abandoned Pb-Zn mining area in Hunan Province, China. Concentrations of Cd, Pb, Zn, Cu, and Ni were measured using Inductively Coupled Plasma Mass Spectrometry (ICP-MS), and As was analyzed by atomic fluorescence spectometry (AFS). The mean concentrations of As, Pb, Cd, and Zn in topsoil were 84.85, 802.58, 6.13, and 689.66 mg/kg, respectively – much higher than Class III of Environmental Quality Standard for soils in China (GB 15618-1995). The most contaminated areas appeared in the vicinity of the mine, discarded smelter, and tailing pond. Heavy metal contents in soils around the tailing pond increased substantially with soil depth, while first increasing and then decreasing with the increased soil depth around the abandoned smelter. The overall potential ecological risks of soil heavy metals in this abandoned mining area were moderate. However, heavy metals in soils around the tailing pond indicated a severe potential ecological risk, which should give rise to widespread concerns.
4

HIF-1α promotes the migration and invasion of hepatocellular carcinoma cells via the IL-8–NF-κB axis

76%
Feng W., Xue T., Huang S., Shi Q., Tang C., Cui G., Guo H.
Cellular and Molecular Biology Letters
|
2018
|
tom 23
Background: Hypoxia plays a critical role in many cancers. Hypoxia inducible factor-1α (HIF-1α) is an important mediator of the hypoxia response. It regulates the expression of various chemokines involved in tumor growth, angiogenesis and metastasis but the associated pathway needs further investigation. Methods: The expression level of HIF-1α was determined in hepatocellular carcinoma (HCC) cells. The correlation of interleukin-8 (IL-8) and HIF-1α was assessed by knocking down HIF-1α. These cells were also used to assess its influence on HCC cell migration and invasion was checked. Pyrrolidinedithiocarbamate (PDTC), an inhibitor of NF-κB, was used to confirm the associated signaling pathway. Results: HIF-1α was significantly expressed in HCC cells and found to promote HCC cell migration and invasion in an IL-8-dependent manner. NF-κB was confirmed to be involved in the process. Conclusions: HIF-1α promotes HCC cell migration and invasion by modulating IL-8 via the NF-κB pathway.
5

Physiological characterization and transcriptome analysis of a chlorosis mutant in pak choi

76%
Ye X., Ren J., Huang S., Chi M., Zhang Y., Feng H., Liu Z.
Acta Physiologiae Plantarum
|
2019
|
tom 41
|
nr 07
Leaf color mutants are not only ideal resources for studying the physiological mechanisms of plant photosynthesis, but they are also selection markers for hybrid production. In this study, the chlorosis mutant ‘564Y’ and its wild-type counterpart ‘564’ were used to explore physiological characteristics and gene expression in pak choi. Compared with those of wild type, the dry weight and fresh weight of ‘564Y’ were significantly reduced, but the hypocotyl length of ‘564Y’ was longer. Pigment analysis showed that the chlorophyll a and carotenoid contents of ‘564Y’ were lower than those of wild type. However, there was no significant difference in chlorophyll b content between these two varieties. The photosynthetic capacity of ‘564Y’ was lower than that of ‘564’, and the photosynthetic electron transfer ability and light energy conversion efficiency were weaker in the former. Transmission electron microscopy showed that the chloroplast structure of ‘564Y’ was not compact, the thylakoid structure was irregular, and the number of matrix, grana, and stacking layers was decreased. Transcriptome analysis revealed 2958 differentially expressed genes (DEGs), and 78 gene ontology terms and 35 Kyoto Encyclopedia of Genes and Genomes pathways were significantly enriched. Among the DEGs, nine genes were associated with the chlorophyll synthesis pathway (Bra031690, Bra012595, Bra005677, Bra022628, Bra026410, Bra032155, Bra039206, Bra029875, and Bra012511) and one gene was associated with photosynthesis (Bra038011). Among these genes, 3 were identified as related to auxin metabolism too (Bra032155, Bra039206, and Bra029875). Their differential expression in the ‘564Y’ mutant and wild-type plant was confirmed by qRT-PCR. These results lay the foundation for future genetic and functional genomic studies in pak choi.
6

The effect of buffalo CD14 shRNA on the gene expression of TLR4 signal pathway in buffalo monocyte/macrophages

76%
Li X., Li M., Huang S., Qiao S., Qin Z., Kang C., Shi D.
Cellular and Molecular Biology Letters
|
2014
|
tom 19
|
nr 4
CD14 plays a crucial role in the inflammatory response to lipopolysaccharide (LPS), which interacts with TLR4 and MD-2 to enable cell activation, resulting in inflammation. Upstream inhibition of the inflammation pathway mediated by bacterial LPS, toll-like receptor 4 (TLR4) and cluster of differentiation antigen 14 (CD14) was proven to be an effective therapeutic approach for attenuating harmful immune activation. To explore the effect of CD14 downregulation on the expression of TLR4 signaling pathway-related genes after LPS stimulation in buffalo (Bubalus bubalis) monocyte/macrophages, effective CD14 shRNA sequences were screened using qRT-PCR and FACS analysis with buffalo CD14 shRNA lentiviral recombinant plasmids (pSicoRGFP-shRNA) and buffalo CD14 fusion expression plasmids (pDsRed-N1-buffalo CD14) co-transfected into HEK293T cells via liposomes. Of the tested shRNAs, shRNA-1041 revealed the highest knockdown efficiency (p < 0.01). When buffalo peripheral blood monocyte/macrophages were infected with shRNA-1041 lentivirus and stimulated with LPS, the expression of endogenous CD14 was significantly decreased by CD14 shRNA (p < 0.01), and the mRNA expression levels of TLR4, IL-6 and TNF-α were also significantly downregulated compared to the control groups (p < 0.01). These results demonstrated that the knockdown of endogenous CD14 had clear regulatory effects on the signal transduction of TLR4 after stimulation with LPS. These results may provide a better understanding of the molecular mechanisms of CD14 regulation in the development of several buffalo diseases.
7

Morphmetric analysis of Apis Cerana populations in Huangshan, China

76%
Yu L., Liu F., Huang S., Bi S., Zong C., Wang T.
Journal of Apicultural Science
|
2013
|
tom 57
|
nr 2
8

Upregulation of CCL3/MIP-1alpha regulated by MAPKs and NF-kappaB mediates microglial inflammatory response in LPS-induced brain injury

64%
Zhu X., Wei D., Chen O., Zhang Z., Xue J., Huang S., Zhu W., Wang Y.
Acta Neurobiologiae Experimentalis
|
2016
|
tom 76
|
nr 4
Growing evidence suggests that macrophage inflammatory protein (MIP)‑1alpha (synonym CCL3) is upregulated in the neuroinflammatory processes initiated by some brain disorders, but its precise role and regulatory mechanism remain unclear. The present work aims to evaluate the role of CCL3/MIP‑1alpha in lipopolysaccharide (LPS)‑induced brain injury, and investigate whether the MAPKs and NF‑kappaB regulate CCL3/MIP‑1alpha expression. We firstly examined the patterns of CCL3/MIP‑1alpha expression and phosphorylation of MAPKs in the brains of rats 6, 24, and 72 h after LPS administration. Additionally, LPS‑treated rats were administered an anti‑MIP‑1alpha neutralizing antibody, and the microglial reaction and the expression of both cyclooxygenase‑2 and inducible nitric oxide synthase (iNOS) were analyzed. We finally evaluated the effect of an inhibitor of P38 MAPK, an inhibitor of ERK1/2, or an inhibitor of NF‑kappaB, on the levels of CCL3/MIP‑1alpha protein and numbers of microglia in the brain. In the observation period, LPS induced CCL3/MIP‑1alpha expression, which localized to OX‑42‑labeled microglia, leading to time‑dependent increases in the phosphorylation of P38 MAPK and ERK1/2. The expression pattern of induced CCL3/MIP‑1alpha was partly consistent with the phosphorylation of MAPKs (P38 MAPK, ERK1/2). Anti‑MIP‑1alpha attenuated microglial accumulation and the upregulation of cyclooxygenase‑2 and iNOS. The inhibition of P38 MAPK, ERK1/2, or NF‑kappaB signaling reduced the induced upregulation of CCL3/MIP‑1alpha and the microglial accumulation. Our data suggest that upregulated CCL3/MIP‑1alpha mediates the accumulation of microglia and the neuroinflammatory reaction, and its expression may be regulated by MAPKs and NF‑kappaB in LPS‑induced brain injury.
9
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
Content available

Selection of reference genes for quantitative real-time RT-PCR on gene expression in Golden Pompano (Trachinotus ovatus)

64%
Chen X. J., Zhang X. Q., Huang S., Cao Z. J., Qin Q. W., Hu W. T., Sun Y., Zhou Y. C.
Polish Journal of Veterinary Sciences
|
2017
|
tom 20
|
nr 3
Golden pompano (Trachinotus ovatus) is an important economically fish species. In this study, with an aim to identify reliable reference genes for quantitative real-time PCR (qRT-PCR) in golden pompano, we evaluated the expression stability of eight housekeeping genes in the presence and absence of poly I:C stimulation in eight tissues. The PCR data was analyzed by geNorm and NormFinder algorithms. The results showed that the expression of all the examined genes exhibited tissue-dependent variations. When under normal physiological condition, geNorm and NormFinder identified B2M and 18S as suitable genes. When studying gene expression under conditions of poly I:C stimulation, the selection of the internal controls should be selected on a tissue basis. At 12 h stimulation, geNorm ranked Actin/UBCE, Actin/B2M, UBCE/B2M, Actin/UBCE, RPL13/B2M, UBCE/GAPDH, B2M/RPL13, and UBCE/B2M, respectively, as the most stably expressed genes in liver, spleen, kidney, gill, intestine, heart, muscle, and brain. Comparable ranking orders were produced by NormFinder. Similar results were obtained at 48 h stimulation. Taken together, these results indicate that B2M and 18S are the most stable gene across tissue types under normal physiological conditions. However, during poly I:C stimulation, no single gene or single pair of genes in the examined set of housekeeping genes can serve as a universal reference across all tissue types. If one gene is preferred, B2M, B2M, UBCE, Actin, B2M/RPL13, B2M, B2M, and RPL13 may be used in spleen, kidney, liver, gill, intestine, brain, muscle, and heart of golden pompano, respectively.
10

The AtDREB1A transcription factor up-regulates expression of a vernalization pathway gene, GmVRN1-like, delaying flowering in soybean

64%
Suo H., Lii J., Ma Q., Ma Q., Yang C. Y., Zhang X. X., Meng X., Huang S., Nian H.
Acta Physiologiae Plantarum
|
2016
|
tom 38
|
nr 06
11

Linkage disequilibrium and functional analysis of PRE1 insertion together with SNPs in the promoter region of IGFBP7 gene in different pig breeds

64%
Wu Q., Yu H., Wei W., Cheng Y., Huang S., Shi H., Liu S., Xia J., Jia H., Hao L.
Journal of Applied Genetics
|
2018
|
tom 59
|
nr 2
12

Genome-wide association study on chicken carcass traits using sequence data imputed from SNP array

64%
Huang S., He Y., Ye S., Wang J., Yuan X., Zhang H., Li J., Zhang X., Zhang Z.
Journal of Applied Genetics
|
2018
|
tom 59
|
nr 3
13

Liposomes as ultrasound imaging contrast agents and as ultrasound-sensitive drug delivery agents

64%
Huang S., Hamilton A. J., Tiukinhoy S. D., Nagaraj A., Kane B. J., Klegerman M., Mcpherson D. D., Macdonald R. C.
Cellular and Molecular Biology Letters
|
2002
|
tom 07
|
nr 2
14

Physiological responses and tolerance of flax (Linum usitatissimum L.) to lead stress

52%
Pan G., Zhao L., Li J., Huang S., Tang H., Chang L., Dai Z., Chen A., Li D., Li Z., Deng Y.
Acta Physiologiae Plantarum
|
2020
|
tom 42
|
nr 07
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