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In vitro regeneration of Fragraria plants

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Fragaria is one of genus in Rosaceae family. The most popular representative is strawberry (Fragaria × ananassa Duch.) and wild (woodland) strawberry (Fragaria vesca L.), which taste attributes are very attractive for a huge number of consumers around the world. The plants have many beneficial traits, such as low-caloric, high amount of antoxidants and vitamin C, laxative, diuretic, astringent, antidiarrheal and antiseptic properties. Cultivation of Fragaria plants is widespread worldwide with particular emphasis on moderate climate zone, also with use of a plant tissue culture method. This thesis showed and contrasted other studies about Fragaria plants propagation under in vitro conditions. In this method the most often used explants are leaf explants. Very rarely are used seeds. Mainly, the plants are propagated on basal medium of mineral composition by Murashige and Skoog in 8 hour dark and 16 hour light contidions. The most efficient cytokinin used to root induction is indole-3-butyric acid (IBA). The plant acclimatization had varying effectiveness – from a few to several dozen survival percent. During micropropagation of Fragaria plants, somaclonal variation occurs, which is dependent on age culture, frequency of passage and medium composition.
The genus Fragaria belongs to the Rosaceae family. The most popular representatives of this species are the strawberry (Fragaria × ananassa Duch.) and wild strawberry (Fragaria vesca L.), whose taste and health benefits are appreciated by a huge number of consumers. The cultivation of Fragaria plants is widespread around the world, with particular emphasis on the temperate climate zone. Increasingly occurring weather anomalies, including drought phenomena, cause immense losses in crop cultivation. The Fragaria plant species are very sensitive to drought, due to the shallow root system, large leaf area and the high water content of the fruit. There have been many studies on the influence of water deficit on the morphological, biochemical and physiological features of strawberries and wild strawberries. There is a lack of research summarizing the current state of knowledge regarding of specific species response to water stress. The aim of this study was to combine and compare data from many research carried out and indicate the direction of future research aimed at improving the resistance of Fragaria plants species to stress related to drought. These plants show patterns of response to stress caused by drought, such as: osmotic adjustment, reduction of transpiration and photosynthesis, and increased efficiency of water use. Drought also causes significant changes in the composition and palatability of the fruit of the Fragaria plant species.
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