Wyniki wyszukiwania

Sortuj według:

Ogranicz wyniki do:

A saponin isolated from Agapanthus africanus differentially induces apoplastic peroxidase activity in wheat and displays fungicidal properties

100%

Cawood M. E., Pretorius J. C., van der Westhuizer J. H., van Heerden F. R.

Acta Physiologiae Plantarum

2015

tom 37

nr 11

A spirostane with an attached trisaccharide, (25R)-5α-spirostane-2α,3β,5α-triol 3-O-(O-α-L-rhamnopyranosyl-( 1 + 2)-O-(β-D-galactopyranosyl-(1 + 3))-β-Dglucopyranoside), was isolated and identified from the aerial parts of Agapanthus africanus by activity-guided fractionation. Fungicidal properties of the crude extract, semi-purified fractions as well as the purified active saponin from A. africanus were screened in vitro against Fusarium oxysporum. At a concentration of 1 mg mL-1, the crude extract and semi-purified ethyl acetate and dichloromethane fractions showed significant antifungal activity. The purified saponin inhibited the in vitro mycelial growth of F. oxysporum completely (100 %) at a concentration of 125 lg mL-1. Furthermore, to verify previously observed induced resistance by crude extracts of A. africanus towards leaf rust, intercellular PR-protein activity was determined in wheat seedlings following foliar application of the purified saponin at 100 lg mL-1. In vitro peroxidase enzyme activity increased significantly (60 %) in wheat seedlings 48 h after treatment with the purified saponin, demonstrating its role as an elicitor to activate a defence reaction in wheat.

Ograniczanie wyników

1 Acta Physiologiae Plantarum

1 Cawood M. E.

1 Pretorius J. C.

1 van Heerden F. R.

1 van der Westhuizer J. H.

1 2015

Wyniki wyszukiwania

A saponin isolated from Agapanthus africanus differentially induces apoplastic peroxidase activity in wheat and displays fungicidal properties