Black Lipid Membrane (BLM) technique was used to examine effects of BSA (Bovine serum albumin) on properties on the model membrane. BLMs were formed with EYPC (L-αPhospatydylocholine from eggs yolk) in n-dekane. BLM studied were performed in phosphate buffer (pH 7.4). Various concentrations of BSA (between 0.01 mg/ml and 2.5×10-3 mg/ml) were used in order to optimize experimental conditions. BSA concentrations above 0.01 mg/ml proved too high as they caused collapse of the lipid membrane. Supplementation of the protein resulted in change of resistance of the lipid membrane. The experiment indicates that the protein incorporates into the lipid membrane.
JavaScript jest wyłączony w Twojej przeglądarce internetowej. Włącz go, a następnie odśwież stronę, aby móc w pełni z niej korzystać.