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There were tested and screened, in vitro, nine plant extracts manufactured by Hofigal S.A., against Fusarium oxysporum (strain Fo 18) isolated from blackcurrant plants (Ribes nigrum L.). The highest in vitro activity (efficacy 78.6%) was recorded for Allium sativum extract, followed by Satureja hortensis and Valeriana officinalis extracts (71.4% efficacy), at 20% concentration. A good inhibitory activity on mycelial growth has been observed for Mentha sp., Rosmarinus officinalis, Hyssopus officinalis and Artemisia dracunculus ‘Sativa’ (62.8%, 58.6%, 57.1% and, respectively, 50% efficacy). Achillea millefolium extract had no effect on radial growth of F. oxysporum isolate. This report is the first in Romania regarding the in vitro antifungal activity of some plant extracts on F. oxysporum in blackcurrant. These data are very useful for plant protection practice, particularly for medicinal plants, as blackcurrant, which demands for non pollutant and environmental friendly alternative methods to fungicides. Locally plant extracts could have important roles in sustainable based management strategies of Fusarium disease in blackcurrant.
There were tested and screened, in vitro and in vivo, for the first time in Romania, nine respectively six plant extracts manufactured by Hofigal S.A. against Botrytis cinerea (strain Bc 27) isolated from blackcurrant (Ribes nigrum L.). The highest antibotrytis in vitro activity (efficiency between 80 and 100%) was obtained using the following extracts: Hyssopus officinalis (at 20, 10 and 5%), Satureja hortensis, Allium sativum, Tagetes patula (at 20 and 10%) and Mentha (at 20%). A moderate antibotrytis activity (efficiency between 35.7 and 65.7%) has been noticed for Mentha (at 10 and 5%), Satureja hortensis, Allium sativum and Tagetes patula (at 5%) extracts. The lowest antibotrytis activity or no efficiency was noticed using extracts obtained from Achillea millefolium, Artemisia dracunculus ‘sativa’, Rosmarinus officinalis and Valeriana officinalis even applied at 20%. Based on results obtained in in vitro tests, six plant extracts were tested and screened in vivo, under field conditions at Hofigal S.A. Bucharest. Satureja hortensis, Allium sativum, Hyssopus officinalis, Menthaand Tagetes patula extracts have been efficient in limiting gray mold severity in blackcurrant applied at 10% compared to untreated control. No in vivo activity was registered for Valeriana officinalis extract. Plant extracts with highly efficiency can be recommended as a non-polluting and environmental-friendly alternative (organic horticulture) in the protection of blackcurrant as medicinal crop against grey mould, the most economically important disease in Europe at present.
There were tested and screened, in vitro and in vivo, for the first time in Romania, nine respectively six plant extracts manufactured by Hofigal S.A. against Alternaria alternata (strain Aa 20) isolated from blackcurrant (Ribes nigrum L.). The highest in vitro activity (efficacy between 70% and 100%) was recorded for Satureja hortensis and Valeriana officinalis extracts at 20 and 10% concentrations. A good inhibitory activity on mycelial growth (efficacy between 54.3 and 88.6%) has been noticed for Allium sativum, Mentha sp. Rosmarinus officinalis, Tagetes patula extracts (at 10 and 20%). No efficacy was noticed for Artemisia dracunculus ‘sativa’. The extract obtained from Hyssopus officinalis inhibited the mycelial growth of A. alternata only at 20%, with 57.1% efficacy. In field trials, Valeriana officinalis and Satureja hortensis extracts have been very effective in limiting Alternaria disease severity in blackcurrant applied at 10%, compared to untreated control. This data confirmed their strong antifungal potential, both in vitro and in vivo. Based on our results, plant extracts with highly efficacy could be an alternative in the protection of blackcurrant as medicinal crop against Alternaria leaf spot and fruit rot especially in organic horticulture system.
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