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Presentation of beta-galactosidase activity secreted by plant cells

100%

Stano J., Micieta K., Grancai D., Blanarikova V.

Herba Polonica

2007

tom 53

nr 1

Extracellular plant β-galactosidase from opium poppy and celandine was detected on agar plates by the presence of dyed zones from 1-naphthyl-β-D-galactopyranoside used as a synthetic substrate. Evaluating the azo-dye zones assessed the degree β-galactosidase activity. No coloration of the agar medium was observed on non-inoculated parts, in medium inoculated with heat – inactivated cells (10 min. at 100oC) or in medium without substrate with all calli tested. On the agar plates with substrate and sterile opium poppy, celandine or gherkin seedlings (2–6 days old) changes in coloration were observed showing the release of β-galactosidase from the roots during germination.

Identification and determination of extracellular saccharase in cell suspension of Chelidonium majus L.

76%

Micieta K., Stano J., Korenova M., Blanarikova V., Fulmekova M., Matejka P.

Herba Polonica

2005

tom 51

nr 1-2

A simple and rapid procedure for identification and determination of extracellular saccha- rase is described, using a culture medium of Chelidonium majus L. (celandine) cell suspension cultures. Sucrose was used as a substrate for determination of the extracellular and intracellular activities of the studied enzyme. The culture medium (without cells) was used for identification and determination of extracellular enzyme activity. Intracellular activity was estimated using cell suspension.

Ograniczanie wyników

2 Herba Polonica

2 Blanarikova V.

2 Micieta K.

2 Stano J.

1 Fulmekova M.

1 Grancai D.

1 Korenova M.

1 Matejka P.

1 2007

1 2005

Wyniki wyszukiwania

Presentation of beta-galactosidase activity secreted by plant cells

Identification and determination of extracellular saccharase in cell suspension of Chelidonium majus L.