The physiological efficiency and mechanisms of action were studied of gonadotropin releasing hormone analogue (GnRH-A) on the reproductive functions of rabbits. Twenty-four female rabbits (Oryctolagus cuniculus) were randomly divided into four groups. Group EG-1, EG-2 and EG-3 were subcutaneously injected with 1.0 mL GnRH-A antigen at 100 μg/mL, 100 μg/mL and 50 μg/mL. EG-2 and EG-3 were re-injected on day 20. CG was a control group. Ovary and uterus samples in each animal were collected aseptically on day 70. The tissue slices were observed under optical and electron microscopy. Serum FSH and LH concentrations were detected using ELISA.GnRHR, FSH-β and LH-β genes were assayed with real time PCR. OCT, PFV, PFT, EET and UWT were measured with Motic system. The results showed that FSH and LH concentrations in EG-2 and EG-3 reached the peak on day 40. EG-2 was higher than EG-1 and CG (P<0.01) and EG-3 (P<0.05). GnRHR in EG-3 was higher than that in EG-1 and EG-2 (P<0.05). FSH-β in EG-1 and EG-3 were lower than that in EG-2. Meanwhile, LH-β in EG-3 was less than in EG-1 and EG-2.The homologies of GnRHR, FSH-β and LH-β sequences were 98%, 100% and 94% respectively,with those reported in National Center of Biotechnology Information (NCBI). OCT in EG-1 and EG-3 thickened with a maxium in EG-3. PFV, PFT, EET and UWT in EGs were larger than that in CG (P<0.05) Primary and secondary follicles in EGs increased and growed quickly. Nucleolus and mitochondria in EGs were enlarged. Zona pellucida and microvillus of the oocytes broadened and lengthened. In conclusion, GnRH-A could increase synthesis and secretion of FSH and LH, and promote the development of ovary and follicles.
JavaScript jest wyłączony w Twojej przeglądarce internetowej. Włącz go, a następnie odśwież stronę, aby móc w pełni z niej korzystać.