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Plants do not have the ability to sense physical properties of metals, e.g. specific weight. The term ‘‘heavy metal’’ was defined mainly by the specific weight of metals. The definition was often connected with the expectation that the substance should be toxic. This definition is not acceptable and also inconsistent in use as already stressed in literature. However, in Plant Sciences, the term is so widely used that it is hardly possible to eliminate it. We suggest instead defining the term in a more unequivocal way. This should be done on the basis of the periodic system of elements. Here, we suggest introducing the following three subgroups forming the group of heavy metals for use in Plant Sciences. 1st subgroup: all transition elements except La and Ac (Transition metals). 2nd subgroup: rare earth elements, subdivided in the series of lanthanides and the series of actinides including La and Ac themselves (Rare earth metals). 3rd subgroup: a heterogenous group p-elements including the metal Bi, the amphoterous oxides forming elements Al, Ga, In, Tl, Sn, Pb, Sb and Po, and the metalloids Ge, As and Te. We suggest using the term ‘‘lead-group’’ for this 3rd subgroup of heavy metals as in Toxicology and Environmental Sciences, Pb is the most prominent representative of this group.
Turions are survival organs of aquatic plants such as the great duckweed {Spirodela polyrhiza). They consist of approximately 50% storage starch (per dry weight) used to support the growth of newly formed sprouts following germination. They could be employed as a good model system for investigations of the storage starch degradation in plants. To induce starch degradation in the plant cells turions must be irradiated for a few days with continuous light absorbed by the plant photoreceptor phytochrome. During such treatment changes in the profile of proteins associated with the starch grain surface have been observed. It was shown by in vitro binding studies that several proteins (α-amylase, starch dikinase R1, ß- amylase) are desorbed from the surface or lose the ability to bind to it. This effect was especially obvious when starch grains from turions irradiated for 4 days (irradiated samples) were compared to those from turions kept in darkness (dark control). A hypothesis was presented that unknown changes in the surface properties of starch grains might be very important in the mechanism of starch degradation, by altering the binding of proteins. The aim of the study was to investigate these properties immediately before and after the start of the starch degradation. Precise structural analysis of the starch grain surface was performed using a non- contact atomic force microscopy (nc-AFM). The grain surface revealed increasing roughness and a reduced density of the structural elements in the samples after irradiation. Two different kinds of randomly organized surface elements were detected by nc-AFM: the one type of a globular structure and the other one more oblong. They could be considered as the carbohydrate lamellas situated in the different way at the starch granule surface. Both were observed to become larger after irradiation. This might be a result of binding of water molecules to the carbohydrate lamellas or bending the surface carbohydrate helices into superhelices by new inter-carbohydrate hydrogen bonds. Such a modification of the starch granule surface could be a consequence of events started by the photoreceptor phytochrome involving starch phosphorylation / dephosphorylation, perhaps mediated by the newly discovered starch dikinase.
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