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1

Specyfika działania systemu antyoksydacyjnego nasienia knura

100%
Orzolek A., Mietelska K., Wysocki P.
Medycyna Weterynaryjna
|
2016
|
tom 72
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nr 05
An excessive generation of reactive oxygen species (ROS) in boar semen leads to a reduced motility and fertilizing ability of spermatozoa. Boar spermatozoa, because of a high content of polyunsaturated fatty acids (PUFAs) in their structure, are highly sensitive to lipid peroxidation (LPO). This process, induced by ROS generation, causes irreversible changes in the conformation and integrity of plasmalemma. The boar’s reproductive system includes a special antioxidant system consisting of enzymatic components and antioxidants of low molecular weight. The most active of antioxidant enzymes present in boar semen is superoxide dismutase (SOD). SOD transforms superoxide anion (O₂˙⁻) into hydrogen peroxide (H₂O₂). Because H₂O₂ can easily diffuse across the membranes, it is most harmful to boar spermatozoa. Given the low content of antioxidants of low molecular weight (e.g. L-gluthatione or L-ergothioneine) and the absence of catalase (CAT) activity in boar semen, there must be other mechanisms responsible for the scavenging of hydrogen peroxide and other ROS. This function is probably accomplished mainly by phospholipid hydroperoxide gluthatione peroxidase (PHGPx), enzymes of tioredoxin (TRX) and peroxiredoxin (PRDX) groups, as well as by paraoxonase type 1 and 2 (PON-1 and PON-2).
2
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Effect of boar ejaculate fraction, extender type and time of storage on quality of spermatozoa

76%
Dziekonska A., Swiader K., Koziorowska-Gilun M., Mietelska K., Zasiadczyk L., Kordan W.
Polish Journal of Veterinary Sciences
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2017
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tom 20
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nr 1
The aim of this study was to investigate the effect the sperm-rich fraction (F1) and the post-F1 fraction (F2) on the quality of boar spermatozoa stored in a liquid state. Ejaculates were collected from three Polish Landrace boars. Each ejaculate fraction was diluted with BTS short-term extender and Safe-Cell Plus (SCP) long-term extender and stored for seven days (D1-D7) at 17°C. Analyses included sperm motility parameters, normal apical ridge (NAR) acrosomes and plasma membrane integrity (PMI). Prior to the dilution of fractions, marked changes (p<0.05) were noted between F1 and F2 in progressive motility (PMOT), velocity average pathway (VAP) and velocity straight line (VCL). After the ejaculate was diluted, the type of fraction and type of extender significantly affected (p<0.05) PMOT, being markedly higher (p<0.05) for F1 extended in BTS. No marked changes (p<0.05) were observed between F1 and F2 extended in SCP for any of the analyzed sperm quality parameters during seven days of storage. Significantly higher (p<0.05) values of sperm quality parameters were noted in F1 compared with F2 for BTS on D7 of storage. The results of the four-way ANOVA analysis indicate that boar, fraction of ejaculate, extender type and day of storage had significant effects on the quality of boar stored spermatozoa. The F1 was characterised by higher quality of spermatozoa during storage in comparison with F2 in the short-term extender. Using the long-term extender containing the proteins allowed for a better application of F2, which could be important for the pig industry.
3
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Effects of the platelet - activating factor (PAF) on selected quality parameters of cryopreserved bull semen (AI) with reduced sperm motility

76%
Lecewicz M., Kordan W., Majewska A., Kaminski S., Dziekonska A., Mietelska K.
Polish Journal of Veterinary Sciences
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2016
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tom 19
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nr 1
The aim of the study was to determine the effects of platelet-activating factor (PAF) on selected quality parameters of cryopreserved bull semen with reduced sperm motility used for artificial insemination. The aim of experiment 1 was to identify the optimal concentration of the phospholipid able to preserve sperm viability. Cryopreserved semen was treated with different PAF concentrations: 1x10-5M, 1x10-6M, 1x10-7M, 1x10-8M and 1x10-9M. The experiment demonstrated that PAF at concentration 1x10-9M increased most the sperm viability parameters (motility parameters, plasma membrane integrity and mitochondrial function) after 120 min of incubation of thawed semen at 37oC. Cryopreserved bull semen with reduced sperm motility (below 70%) was supplemented with PAF in a concentration of 1x10-9M. A statistically significant increase in sperm motility, percentage of linear motile spermatozoa and VSL value was observed after 120 min incubation of sperm with 1x10-9M PAF. Sperm supplementation with PAF also had positive effects on plasma membrane integrity and percentage of spermatozoa with preserved mitochondrial transmembrane potential, but the differences were not statistically significant. The results indicated positive effects of PAF supplementation at a concentration of 1x10-9M on the selected sperm quality parameters in cryopreserved bull semen with reduced motility.
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