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Pinus eldarica has been introduced to Iran from more than 800 years ago. Some individuals of this pine have altered both in shape and growth rate in northeastern of Iran, and generated two distinct morphotypes which are called Conical-shaped and Ball-shaped pines. This study was conducted to discriminate these morphotypes using macromorphological and anatomical characteristics. Results of macromorphological analysis showed significant differences both with univariate and multivariate analysis and consequently two new morphotypes were clearly separated from Mondell pine individuals. Furthermore, anatomical differences observed in Conical-shaped pine in comparison with two other pines, from some valuable taxonomical point of view traits such as cross-section form of needle, number of stomata per area, number and position of resin ducts etc. Furthermore, the existent difference in traits like cuticle thickness, stomata density, needle perimeter and length, state increasing the adaptation potential to aridity in Conical-shaped pine in comparison with two others. The differences of two new morphotypes demonstrate that they are new variants of Mondell pine and it is need to be used molecular markers and phylogenic studies for specifying the cause of these morphological and anatomical differences.
Induction of antioxidant systems of hazel cells by low-energy ultrasound, the potential role of hydrogen peroxide (H2O2) as a signaling molecule in regulation of activity of stress-related enzymes, and expression of catalase (CAT) and phenylalanine ammonialyase (PAL) genes were investigated. Suspension-cultured Corylus avellana L. cells were agitated by an ultrasonic device at 29 kHz with the power of 4 mW/cm2, for 8–40 min. The activities of CAT, superoxide dismutase (SOD), and ascorbate peroxidase (APX) of treated cells increased by 4, 1.7 and 7 times of the control ones, respectively. Induction of increase in the expression of CAT gene started 24 h after the treatment with ultrasound. Significant increase also was observed in the expression of PAL gene, 6 h after exposure to ultrasound, which resulted in turn to increase of total contents of soluble phenolics, 24 h of the treatment. Exposure to ultrasound up to 20 min had no adverse effects on cell viability although it slightly increased the accumulation of H2O2. However, it is likely that this level of increased H2O2 was not deteriorative for hazel cells, but rather triggered antioxidant system and provided hazel cells a sustainable growth after ultrasound treatment.
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