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The hitherto investigations of intestinal microflora of mammals at application of diagnostic bacteriological methods have proved a wide diversity of intestinal microflora and of its distribution in particular alimentary tract sectors depending on microflora and animal kind (4). In white rats no living E. coli cells in stomach and forepart of small intestine have been found, probably in connection with too acid reaction. It is in accordance with own observations made in previous works. On the other hand, a question arises, whether E. coli cells taken up by an animal with fodder or in another way, and submitted to action of gastric juice, will be digested in further alimentary tract parts and whether a danger existe of release into intestine of higher endotoxin amounts. This question constitutes just the subject of the present work.
Physiological levels of zinc in the liver, spleen, pancreas, kidney, thymus, blood, plasma and serum were investigated in 25 slaughtered cattle during summer and winter seasons. The investigations were carried out by the method of activaiion analysis using incinerated samples irradiated with thermal neutrons. In the summer season the following zinc levels were found: the blood 0.24, p1asma 0.125, serum 0.126 mg per 100 ml and the liver 3.42 spleen, 2.32, pancreas 2.61, and kidney 1.9 mg per 100 g of the organ. In the winter season these values were as follows: the blood 0.20, plasma 0.123, serum 0.09 mg/100 ml; and the liver 2.85, spleen 2.02, pancreas 2.25 and kidney 1.9 mg per 100 g of the organ. In the summer season the zinc levels were usually higher.
The aim of the work was to detect in different tissues of hens the trace elements activated by irradiation with a stream of thermic neutrons, for determining their mutual qualitative and quantitative ratio in particular tissues. In view of too high activity for transport from the reactor, caused by radiation of isotopes with a short half-life, the samples had to be stored for 3 months for cooling, hence the quality of radioactive elements suitable for investigation had to be limited to those of long-life and emitting the gamma and beta rays, i.e. in the given case to caesium, zinc and cobalt.
The present investigations were aimed at recognition of the metabolism of caesium in the muscular tissues, with the purpose of determining a selective tissue, optimally suitable for investigations on radioactive contamination. An impulse to the investigations have given the results of previous tentative experiments suggesting an unequal distrihution in the muscular tissues of cattle.
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