Microstoma protracta (Fr.) Kanouse is a strictly protected species in Poland, which is also present on the red list with the category R (rare). In Poland, there are five known localities for the species, including four historical sites discovered at the beginning of the twentieth century and one contemporary site discovered at the end of the twentieth century. In this paper, we present two new localities for M. protracta, discovered in April 2006 in Czeszewo (Wielkopolska Lowland, Jarocin Forest District) and in April 2017 in Krakow-Tyniec (Krakow-Częstochowa Upland, Myślenice Forest District). Sequences of the nuclear ribosomal DNA internal transcribed spacer regions of sporocarps from contemporary localities have been deposited in the GenBank database. This is the first record of the DNA barcode for this species. According to Criterion D of the IUCN recommendations, it has been suggested that M. protracta should be considered as a critically endangered species (CR: D) in Poland.
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