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Nitrogen deficiency stimulates biosynthesis of bioactive phenylethanoid glycosides in the medicinal plant Castilleja tenuiflora Benth

100%

Medina-Perez V., Lopez-Laredo A. R., Sepulveda-Jimenez G., Zamilpa A., Trejo-Tapia G.

Acta Physiologiae Plantarum

2015

tom 37

nr 05

Castilleja tenuiflora Benth. (Orobanchaceae) in vitro cultures are alternative sources of phenylethanoid glycosides (PhGs), promising natural products for chronic diseases treatment because of their extensive range of biological activities. To increase the yields of these bioactive compounds, the identification of factors affecting their biosynthesis is required. Here, we show that N deficiency stimulates PhGs biosynthesis by increasing the activity of PAL. The contents of two PhGs, verbascoside and isoverbascoside, were enhanced under N deficiency compared with control conditions. The maximum PAL activity in shoots cultured under N deficiency was also increased. Furthermore, we found de novo anthocyanin synthesis under N deficiency suggesting an additional impact of this stress factor on flavonoid metabolism. N deficiency negatively affected the shoot growth (length, biomass, and total chlorophyll content) and multiplication rate, and inhibited the root formation of C. tenuiflora shoots. Our results demonstrate that N deficiency increases PhGs biosynthesis in C. tenuiflora.

Variation in antioxidant properties and phenolics concentration in different organs of wild growing and greenhouse cultivated Castilleja tenuiflora Benth.

86%

Lopez-Laredo A. R., Gomez-Aguirre Y. A., Medina-Perez V., Salcedo-Morales G., Sepulveda-Jimenez G., Trejo-Tapia G.

Acta Physiologiae Plantarum

2012

tom 34

nr 6

The content of total phenolic compounds and flavonoids was determined in methanol extracts of root, stem, leaves, and inflorescences from wild growing and greenhouse cultivated plants of Castilleja tenuiflora. The antioxidant activity in each extract was evaluated using three in vitro models: scavenging of free radicals with 2, 2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) diammonium salt (ABTS), and reducing power by the phosphomolybdenum assay. Both, antioxidant activity and phytochemicals content were influenced significantly (P<0.05) by the source of the plant material and the organ. Cultivated plants had the highest content of phenolic compounds (37.95 mg gallic acid equiv. g⁻¹ dry weight, P<0.05) and the strongest antioxidant activity. Total phenolic compounds content correlated significantly with the antioxidant activity for all studied plant material and organs (P<0.05). TLC profile using DPPH as a detection reagent indicated that the phenylethanoids verbascoside and isoverbascoside are the main contributors to the free-radical scavenging of C. tenuiflora. Cultivated plants of C. tenuiflora are an alternative source of natural antioxidants to wild growing plants. The antioxidant properties of C. tenuiflora may be associated with its traditional use to treat conditions consistent with radical-related diseases (e.g. Inflammation, tumors).

Ograniczanie wyników

2 Acta Physiologiae Plantarum

2 Lopez-Laredo A. R.

2 Medina-Perez V.

2 Sepulveda-Jimenez G.

2 Trejo-Tapia G.

1 Gomez-Aguirre Y. A.

1 Salcedo-Morales G.

1 Zamilpa A.

1 2015

1 2012

Wyniki wyszukiwania

Nitrogen deficiency stimulates biosynthesis of bioactive phenylethanoid glycosides in the medicinal plant Castilleja tenuiflora Benth

Variation in antioxidant properties and phenolics concentration in different organs of wild growing and greenhouse cultivated Castilleja tenuiflora Benth.