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The aim of this study was to evaluate how training affects plasma total, free and esterified L-carnitine concentrations in horses. The study was done on a total of 32 healthy, trained purebred Arabian horses. The 32 horses comprised two groups. In the first group, 12 stallions and 6 mares three-year-old involved in race-training were studied at rest. In the second group, 14 stallions were studied during a typical race or endurance training session. Jugular venous blood samples were collected to determine lactic acid (LA), free fatty acids, glycerol, triacylglycerides (TG), and the total esterified and free L-carnitine contents. The race training session caused a significant increase in blood LA and plasma esterified L-carnitine, glycerol, and TG levels. It also caused a decrease in the plasma free L-carnitine concentration determined just after the end of exercise. Compared with endurance training, high-intensity racing exercise increased the plasma esterified L-carnitine concentration and decreased that of free L-carnitine according to training intensity.
This study tests the hypothesis found in professional literature that increased plasma creatine kinase (CK) activity in horses is a direct result of a rise in the plasma glucose level. Participants were 24 three-year-old Purebred Arabian mares and 67 three to six-year-old Purebred Arabian stallions. From the total of 91 horses, 29 of the horses were studied only once and 62 were studied two, three or four times. As a result, the total number of studied exercise tests amounted to 176. All horses were being tested whilst undergoing race training. On the days of the study, the horses took part in a conventional training session. Three blood samples from the jugular vein were collected from each horse. These samples were taken while 1) at rest, 2) after the end of training session and 3) thirty min. after the end of effort. In the obtained plasma samples, glucose level and CK activity were determined using spectrophotometric diagnostic kits. The exercise regime which the studied horses had undertaken evoked a statistically significant increase in both means of the analyzed parameters. An analysis of results using a coefficient correlation indicated that there was no correlation between the changes in plasma glucose level and CK activity. The present study does not seem to support the hypothesis of increased plasma CK activity in horses being the result of a rise in the plasma glucose level.
The study discusses molecular mechanisms of the action of estrogens (E) in the brain and their effect on sexual behavior in females. E increase the synthesis of the neuromediators: oxytocin and dopamine in the brain. E also influence K+ channel activity in neurons. Specific intracellular E receptors exist in the neurons. These receptors bind E and then activate a regulatory mechanism dependent on the protein G complex and on the cyclical AMP response element binding proteins (CREB). As a result, synaptic activity of the neurons increase and the expression of the brain-derived neurotrophic factor (BDNF) gene also take place. Moreover, E stimulate the synthesis of the sex hormone binding globulin (SHBG). SHBG plays a role as a steroids transporter and also as a signaling substance in the brain.
The effect of interval vs. continuous exercise on plasma leptin and ghrelin concentration in young Standardbred horses was studied. The experiment was conducted on 27 trotters, in the age between 2 and 3 years. They were divided into two groups according to the type of exercise. Blood samples were collected through jugular venipuncture in the following experimental conditions: at rest, immediately after exercise and 30 minutes after the end of the effort. Plasma leptin and ghrelin concentrations were determined using RIA tests. The continuous exercise induced an increase in plasma leptin concentration whereas the interval type of exercise did not influence the level of this hormone (3.47 ± 0.78 vs. 4.07 ± 0.94 and 2.31 ± 0.15 vs. 2.36 ± 0.21 ng/mL, respectively). The plasma ghrelin concentration measured after the continuous exercise, significantly increased (720 ± 27.4 vs. 814 ± 13.8; p ≤ 0.05) whereas concentration of this hormone assessed after the interval exercise, significantly dropped (982 ± 56.5 vs. 842 ± 35.6 pg/mL; p ≤ 0.05). The changes in plasma ghrelin concentration measured after the end of the effort correlated inversely with blood lactic acid concentration. In conclusion, the obtained results showed that medium-intensive type of exercise, such as trot, interval or continuous, slightly affected plasma leptin level but significantly affected plasma ghrelin concentration in young Standardbred trotters.
Robert Koch, a German Nobel Prize winner, said a remarkable statement a century ago: „Time will come when the man will curb noise as he fights cholera and plague today“. Above 40% Poland’s population live exposed to noise above permissible levels. In other countries situation is similar. It can be supposed that the Koch’s statement is about to come true. The article provides a sufficient number of arguments which justify such a standpoint.
The study explored the hypothesis that mares, which are more sensitive than stallions, react to stress with higher increase in heart rate (HR).A group was studied of 101 clinically healthy Standardbred trotters aged 11-18 months, during their daily training routine. The horses were divided into two groups according to sex (51 colts and 50 fillies). All the horses took part in a standard training session consisting of harnessing to the sulky, moving from the stable and a 45 minutes exercise on a sand track. Before the start of training session a belt with a transmitter for telemetric HR registration was placed on each horse. The measured HR did not show any differences in HR between colts and fillies either at rest, during handling and harnessing as well as during exercise and after it.
The aim of this study was to measure emotional arousal in young race horses as expressed by heart rate (HR) during routine situations of grooming, hoof cleaning, and while at rest, having been transported from the environment of home stud farm to that of an unfamiliar training centre. A hypothesis was developed that the change of environment is a source of heavy stress for young race horses. The investigation involved 22 Purebred Arabian stallions and 19 Purebred Arabian mares, 2.5 years old, and 11 Thoroughbred stallions and 10 Thoroughbred mares, at the age of 1.5 years. The horses were examined twice: first in their home stud farms, and then three days after being moved to an unfamiliar training centre. The HR was measured at rest and while the horses were being groomed in their boxes. The HR registered during the grooming session at the new stable increased significantly in all studied groups of horses compared to the data obtained at their home stud farm. This increase was significantly higher in the groups of 1.5-year–old Thoroughbred stallions and mares than in the groups of 2.5-year-old Purebred Arabian horses. Young race horses should not be groomed just before training because the grooming routine involves their emotional arousal.
The objective of the present study was to compare changes of blood parameters induced by the same work and performed in the same conditions in two racehorse breeds, Thoroughbred and Purebred Arabian. The effect of moderate-intensity exercise was studied in 20 stallions - ten Thoroughbreds, aged 2-3 years and the same number of Purebred Arabians, 3-4 years old. All the horses were administrated the same effort test consisting in 1200 m gallop at a speed typical of the daily training sessions. Three jugular venous blood samples were collected for each horse: at rest, just after the end of the gallop and after 30-minute rest. In the gathered blood, a hemoglobin (Hb) concentration was determined as well as plasma level of glucose (Gic), triacylglycerols (TG), glycerol, free fatty acids (FFA), total plasma proteins (TP) and the activity of creatine kinase (CK), lactate dehydrogenase (LDH) and aspartate aminotransferase (AST). In the Arabian horses, an increase in levels of TP, glycerol, FFA and CK activity measured just after exercise was higher than that in Thoroughbreds. Similarly, after a 30-minute rest, a post-exercise rise of TP, AST, glycerol and FFA proved to be higher in the Arabian horses compared to that in the Thoroughbreds. Only TG plasma concentration measured 30 minutes following the effort was significantly lower in the Arabian horses than in Thoroughbreds. It can be concluded that the Thoroughbred horses adapted better to the effort test applied in this study as compared to the Purebred Arabian horses. The parameters related to lipid metabolism proved to be the most sensitive indicators of breed differences in relation to moderate-intensity exercise.
There is not much research done on the influence of sympathetic training on the emotional reaction of horses. The aim of the present study was to evaluate the emotional response and the stress level in horses to two sympathetic training methods: (1) with the use of the “round pen technique” (RP),and (2) in which the RP was not applied (SH). Twenty two naive half-bred Anglo-Arab horses (2.5 years ±3 months of age) were subject to an initial training. Eleven horses were randomly included to the RP method and the other 11 horses for the SH method. Heart rate (HR) and saliva cortisol concentration were measured as indicators of horse emotional arousal and stress level, respectively.The HR values were analysed: at rest, during the habituation period, just after the first saddling and tightening of the girth, during the first time a human leaned over the horse’s back, and during the mounting of the horse. Saliva samples were taken before and 15 min after each training session studied. After saddling, the HR occurred significantly higher when the RP technique was used. The significant increase in saliva cortisol concentration was observed only after the first mounting of naive the horse. Generally, the use of the RP technique did not involve more important physiological reactions in the trained horses than did the SH method.
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