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Production of cellulases by Bacillus cellulosilyticus using lignocellulosic material

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Maryam B., Qadir A., Zameer M., Ahmad S. R., Nelofer R., Jamil N., Arzoo S., Afzaal R.

Polish Journal of Environmental Studies

2018

tom 27

nr 6

The enzyme cellulase, a multi enzyme complex made up of several proteins, catalyses the conversion of cellulose to glucose in an enzymatic hydrolysis. In this study biomass alkali-pretreatment followed by enzymatic hydrolysis was carried out using crude cellulase enzyme produced from screened bacillus strain (Bacillus cellulosilyticus) having 1,998.79 IU/ml/min CMCase and 1,621.16 IU/ml/min FPase enzyme activity. The production of cellulase enzyme using an economical medium has been a significant achievement in the field of industrial biotechnology. The maximum yield of sugars in the form of total sugars (179.84±0.2 mg/ml), reducing sugars (126.72±0.1 mg/ml), and glucose (105.40±0.1 mg/ml) was achieved at 48 h incubation time, 50ºC, 5 pH, 6% enzyme concentration, 4% substrate loading, and PEG 3350 as a surfactant.

Ograniczanie wyników

1 Polish Journal of Environmental Studies

1 Afzaal R.

1 Ahmad S. R.

1 Arzoo S.

1 Jamil N.

1 Maryam B.

1 Nelofer R.

1 Qadir A.

1 Zameer M.

1 2018

Wyniki wyszukiwania

Production of cellulases by Bacillus cellulosilyticus using lignocellulosic material