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1

Wirus jamkowatości pnia jabłoni (ASPV) – występowanie, wykrywanie i charakterystyka molekularna

100%
Komorowska B.
Postępy Nauk Rolniczych
|
2010
|
tom 62
|
nr 1
2

The attempts to produce antiserum against apple stem pitting virus coat protein [ASPV CP] obtained in prokaryotic and eukaryotic expression systems

63%
Komorowska B., Malinowski T.
Journal of Fruit and Ornamental Plant Research
|
2009
|
tom 17
|
nr 2
21-30
Cloning and expression of the coat protein (CP) gene of Apple stem pitting virus (ASPV; Family: Flexiviride, Genus: Foveavirus) using in vivo Escherichia coli and Pichia pastoris as well as in vitro wheat germ extract gene expression system are described. The coat protein gene was amplified for five ASPV isolates representing three groups characterized by a different size of CPs. The amplified RT-PCR products were cloned into bacteria and yeast expression vectors. Plasmid containing the CP gene of the isolate MT32 was used as a template for expression in wheat germ extract. Proteins of expected sizes, reacting with ASPV antiserum in Western blotting ex­periments, were obtained in three expression systems for the different virus isolates used in this study. The purified proteins were used for immunization and preparation of rabbit polyclonal antisera. The obtained antibodies could be used for immunocap- ture RT-PCR detection of ASPV in woody tissue, but ELISA results were not satis­factory.
3

Choroby fitoplazmatyczne w uprawie gruntowej roslin ozdobnych; Wystepowanie, metody wykrywania i identyfikacji

51%
Kaminska M., Malinowski T., Komorowska B., Rudzinska-Langwald A.
Progress in Plant Protection
|
1997
|
tom 37
|
nr 2
363-368
4

The use of quantitative PCR with high resolution melting (qPCR-HRM) analysis to distinguish the Polish mild and yellowing pathotypes of Pepino mosaic virus (PepMV)

51%
Minicka J., Komorowska B., Hasiow-Jaroszewska B.
Progress in Plant Protection
|
2020
|
tom 60
|
nr 1
5
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
Content available

Year-round blueberry scorch virus detection in highbush blueberry

51%
Paduch-Cichal E., Chodorska M., Kalinowska E., Komorowska B.
Acta Scientiarum Polonorum. Hortorum Cultus
|
2014
|
tom 13
|
nr 3
Viral diseases are a worldwide problem of blueberry which a major limiting factor for production. A survey for Blueberry scorch virus (BlScV) by DAS-ELISA in various organs of highbush blueberry conducted from May 2010 to April 2011, showed the occurrence of these virus in cvs Bluecrop and Herbert, which showing virus-like symptoms. Samples of plant materials (bud flower, flower, leaf, bark) were collected individually from each highbush blueberry plant of every cultivar. It was established that the detection of virus of each the investigated bushes cvs Bluecrop and Herbert depended on the tested plant materials as well as the period in which the tests were performed. The effectiveness of the virus detection varied for the investigated cultivars. The presence of the BlScV was confirmed in leaves samples with specific primer pair which amplifies a 430 bp fragment of the 5’-proximal ORF I [RNA-dependent RNA polymerase (RdRp)].
6

The fine structure of the curticle in Tetrodondophora bielanensis (Waga), Collembola. I

51%
Krzysztofowicz A., Klag J., Komorowska B.
Acta Biologica Cracoviensia. Series Zoologia
|
1972
|
tom 15
|
nr 1
7
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
Content available

Detection of Narcissus latent virus isolates using one-step RT-PCR assay

51%
Berniak H., Komorowska B., Sochacki D.
Journal of Horticultural Research
|
2013
|
tom 21
|
nr 1
8

Stunting and flower buds deficiency of Lilium sp.: a new phytoplasma associated disease

51%
Kaminska M., Korbin M., Komorowska B., Pulawska J.
Acta Physiologiae Plantarum
|
1998
|
tom 20
|
nr 1
The presence of phytoplasmas in Lilium sp. showing severely stunted growth, leaf malformation and flower buds deficiency was demonstrated for the first time using polymerase chain reaction assays with primers amplifying phytoplasma 16S rDNA regions. These phytoplasmas were found in leaves as well as roots and bulb scales of symptomatic and CMV and/or LSV affected and asymptomatic virus-free lilies.
9

Isolation, cloning and sequencing of nucleoprotein gene of gerbera isolate of tomato spotted wilt virus [TSWV]

51%
Korbin M., Komorowska B., Wawrzynczak D.
Phytopathologia Polonica
|
2003
|
tom 27
10

Amplification of the coat protein gene of apple stem pitting virus [ASPV]

51%
Komorowska B., Malinowski T., Zawadzka B.
Phytopathologia Polonica
|
1999
|
tom 17
11

Some molecular properties of several isolates of apple chlorotic leaf spot virus from Bulgaria

51%
Cieslinska M., Borisova A., Komorowska B.
Journal of Fruit and Ornamental Plant Research
|
2007
|
tom 15
12

Application of DAPI staining and PCR amplification of DNA for the identification of pear decline phytoplasma in declining trees in Poland

51%
Malinowski T., Zandarski J., Komorowska B., Zawadzka B.
Phytopathologia Polonica
|
1996
|
tom 12
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