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Micropropagation of Cymbidium sinense using continuous and temporary airlift bioreactor system

100%

Gao R., Wu S.-Q., Piao X.-C., Park S.-Y., Lian M.-L.

Acta Physiologiae Plantarum

2014

tom 36

nr 01

Airlift bioreactors were programmed for continuous and temporary immersion culture to investigate factors that affect the rhizome proliferation, shoot formation, and plantlet regeneration of Cymbidium sinense. During rhizome proliferation, the continuous immersion bioreactor system was used to explore the effects of activated charcoal (AC) in the culture medium, inoculation density, and air volume on rhizome differentiation and growth. The optimum conditions for obtaining massive health rhizomes were 0.3 g l⁻¹ AC in the culture medium, 7.5 g l⁻¹ inoculation density, and 150 ml min⁻¹ air. In addition, the temporary immersion bioreactor system was used for both shoot formation and plantlet regeneration. Supplementing 4 mg l⁻¹ 6-benzylaminopurine and 0.2 mg l⁻¹ naphthalene acetic acid (NAA) to the culture medium promoted shoot induction from the rhizome. Cutting the rhizome explants into 1 cm segments was better for massive shoot formation than cutting into 0.25 and 0.5 cm explant segments. NAA promoted plantlet regeneration and the rooting rate (94.7 %), with whole plantlets growing well in culture medium containing 1.0 mg l⁻¹ NAA. Therefore, applying bioreactors in C. sinense micropropagation is an efficient way for scaling up the production of propagules and whole plantlets for the industrial production of high-quality seedlings.

Several factors affecting hypericin production of Hypericum perforatum during adventitious root culture in airlift bioreactors

100%

Wu S.-Q., Yu X.-K., Lian M.-L., Park S.-Y., Piao X.-C.

Acta Physiologiae Plantarum

2014

tom 36

nr 04

Hypericum perforatum L. is a traditional medicinal plant for the treatment of depression and wound healing, and hypericin is one of the main effective active substances. To optimize the culture system for producing hypericin in adventitious root, this study used balloon-type airlift bioreactors to investigate the effect of air volume, inoculation density, indole-3-butyric acid (IBA) concentration and methyl jasmonate (MeJA) concentration on hypericin content and productivity during adventitious root culture. Hypericin content and productivity were improved with increasing air volume, and 0.1 vvm (air volume/culture volume/min) was optimal for hypericin production. Inoculation density also had a great effect on hypericin accumulation. Hypericin content and productivity were favorable in an inoculation density of 5.0 g l⁻¹ and decreased when inoculation densities were lower or higher than 5.0 g l⁻¹. Furthermore, 1.25 mg l⁻¹ IBA enhanced hypericin content and productivity, but too low (≤0.50 mg l⁻¹) or too high (≥1.50 mg l⁻¹) IBA concentrations decreased hypericin accumulation. MeJA concentration significantly affected biomass accumulation and hypericin production. The biomass decreased and hypericin production increased with increasing MeJA concentration. Optimum hypericin content (1.61 mg g⁻¹ DW) and productivity (15.57 mg l⁻¹) were obtained at 350 μM MeJA. The hypericin content in bioreactor-grown adventitious roots was lower than in 3-year field-grown plants, but significantly higher than that in in vitro-grown plantlets and 1-year field-grown plants. Thus, the bioreactor culture of adventitious roots can realize rapid and mass production of hypericin in H. perforatum.

Ograniczanie wyników

2 Acta Physiologiae Plantarum

2 Lian M.-L.

2 Park S.-Y.

2 Piao X.-C.

2 Wu S.-Q.

1 Gao R.

1 Yu X.-K.

2 2014

Wyniki wyszukiwania

Micropropagation of Cymbidium sinense using continuous and temporary airlift bioreactor system

Several factors affecting hypericin production of Hypericum perforatum during adventitious root culture in airlift bioreactors