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An introduction to DNA chips: principles, technology, applications and analysis

100%

Gabig M., Wegrzyn G.

Acta Biochimica Polonica

2001

tom 48

nr 3

This review describes the recently developed GeneChip technology that provides efficient access to genetic information using miniaturised, high-density arrays of DNA or oligonucleotide probes. Such microarrays are powerful tools to study the molecular basis of interactions on a scale that would be impossible using conventional analysis. The recent development of the microarray technology has greatly accelerated the investigation of gene regulation. Arrays are mostly used to identify which genes are turned on or off in a cell or tissue, and also to evaluate the extent of a gene's expression under various conditions. Indeed, this technology has been successfully applied to investigate simultaneous expression of many thousands of genes and to the detection of mutations or polymorphisms, as well as for their mapping and sequencing.

The Escherichia coli RNA polymerase alpha subunit and transcriptional activation by bacteriophage lambda CII protein

61%

Gabig M., Obuchowski M., Ciesielska A., Latala B., Wegrzyn A., Thomas M. S., Wegrzyn G.

Acta Biochimica Polonica

1998

tom 45

nr 1

Bacteriophage λ is not able to lysogenise the Escherichia coli rpoA341 mutant. This mutation causes a single amino acid substitution Lys271Glu in the C-terminal domain of the RNA polymerase α subunit (αCTD). Our previous studies indicated that the impaired lysogenisation of the rpoA341 host is due to a defect in transcriptional activation by the phage CII protein and suggested a role for αCTD in this process. Here we used a series of truncation and point mutants in the rpoA gene placed on a plasmid to investigate the process of transcriptional activation by the CII gene product. Our results indicate that amino-acid residues 265, 268 and 271 in the α subunit may play an important role in the CII-mediated activation of the pE promoter (most probably residue 271) or may be involved in putative interactions between αCTD and an UP-like element near pE (most probably residues 265 and 268). Measurement of the activity of pE-lacZ, pI-lacZ and paQ-lacZ fusions in the rpoA+ and rpoA341 hosts demonstrated that the mechanism of activation of these CII-dependent promoters may be in each case different.

Ograniczanie wyników

2 Acta Biochimica Polonica

2 Gabig M.

2 Wegrzyn G.

1 Ciesielska A.

1 Latala B.

1 Obuchowski M.

1 Thomas M. S.

1 Wegrzyn A.

1 2001

1 1998

Wyniki wyszukiwania

An introduction to DNA chips: principles, technology, applications and analysis

The Escherichia coli RNA polymerase alpha subunit and transcriptional activation by bacteriophage lambda CII protein