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Pseudomonas syringae pv. syringae (Pss) constitutes a diverse group of bacterial strains that cause canker of stone fruits, blight of cereals and red streak of sugarcane. The purpose of this study was to determine how diverse Iranian strains of Pss are when they come from different hosts. We compared a total of 32 Pss strains isolated from stone fruits, barley, wheat and sugarcane from different geographical regions of Iran based on their phenotypic and molecular properties. Strains showed some variation regarding carbon and nitrogen utilization. Pss strains were similar in their protein banding patterns. Additional bands were found in sugarcane strains. Most strains showed one indigenous plasmid DNA and a few had two and some none. The genes of syrB and syrD encoding syringomycin synthesis and secretion, respectively, were amplified using specific primers in polymerase chain reaction. Syringomycin, producing strains amplified two DNA fragments of 752 and 446 bp representing syrB and syrD genes, respectively. Primer specificity was shown for Pss using various genera. Based on the results of this study, it is suggested that Pss strains from different hosts and geographical regions show diversity in phenotypic and molecular characters. It is thought that phenotypic variation is due to adaptation to specific hosts and niches for survival and pathogenicity.
The effects of different doses of date palm (Phoenix dactylifera) seed (DPS) extract on heavy metals (Pb, Cd, As, Hg) concentrations of common carp (Cyprinus carpio) were evaluated. The fish (31.6±3.7 g) were assigned to five treatments of three replications each. Five treatments were held using five different DPS extracts at doses of 0% (control), 0.5%, 1%, 2%, and 4%. The results revealed that heavy metals concentrations of fish decreased in the 0.5% and 1% treatments (P<0.05). Hg and Cd concentrations in muscle decreased in all treatments (P<0.05). The results of this research clearly show that the addition of DPS extract to a fish diet has a significant effect on heavy metals concentrations (P<0.05). DPS extracts were shown to berich in phenolics and fl avonoids. Therefore, they have also been discussed as protectants against heavy metal stress. It could be concluded that feeding common carp a diet enriched with DPS extract could decrease heavy metals concentrations that can cause cancer.
Pesticides along with with their degradation products spread throughout the environment and contaminate the water, leading to a consequent potential risk to humans and also the environment. The study aims to determinene the residual pesticides (organophosphorus, pyrethroids ), herbicides, and fungicides in the water resources (wells, deep wells, springs, spring-heads, and flumes) of Kermanshah Province, Iran. Water samples were taken from 50 stations from April to June 2016, and analyses were performed using gas-chromatography–mass-spectrometry techniques. Data were analysed using SPSS version 20. One of the most important points of this study is the fact that all water resources were safe except for deep wells. Total concentration of organophosphorus was 2.2 ppb. The mean concentration of pyrethroids in Islamabad Gharb and Songhor was 0.574 and 2.235 ppb, respectively. Organophosphorus and pyrethroid pesticides total concentration was more than the European standard. The contamination range of difenoconazole fungicide was 0.024 ppb (Gilan Gharb) and 1.089 ppb (Kermanshah). Glyphosate herbicide was only seen in Islamabad Gharb (0.011 ppb). The mean concentration of all pesticides in the investigated wells was more than the European standard. Estimating pesticide concentration is important for preserving aquatic ecosystems and human health.
An oil refinery is one industry that introduces large amounts of toxic phenol in receiving waters annually. Hence, the aim of this study was phenol removal from oil refinery wastewater by a natural treatment system of a stabilization pond. In this study, a pilot-scale pond volume, hydraulic load, and hydraulic retention time were 200 L, 40 L/day and five days, respectively. Influent concentration of phenol was 100-400 mg/L. Responses of the process were NH₃, PO₄, phenol, TCOD, SCOD, TBOD, SBOD, and pH. The results showed that increasing phenol concentration and decreasing temperature have negative effects on system efficiency. Maximum removal of NH₃, PO₄, phenol, TCOD, SCOD, TBOD, and SBOD were 61.08, 70.09, 93.58, 80.18, 78.89, 78.7, and 76.84% in high temperature, respectively. Maximum efficiency of wastewater treatment system was obtained in phenol concentration at 100 mg/L. Also, anaerobic stabilization ponds are cost-effective options with simple operation that can be employed for the treatment of phenol content of oil refinery wastewaters.
Vegetables are the main source of nitrate food contamination. More than 80% of nitrates are consumed by humans through vegetables. The present study was conducted to investigate the contamination of nitrite and nitrate vegetables in Iran. In this systematic overview we reviewed all internal and external databases for relevant articles. According to different studies, contamination was detected in different types of vegetables in different regions of Iran. Articles that examined nitrite values above the standard limit were not reported. Due to nitrate contamination in vegetables in some cities of Iran and the effects of this nitrogen combination on human health, it is necessary to find a solution in this regard.
Background: The vermiform appendix is a worm-like tube containing a large amount of lymphoid follicles. In our knowledge, there is a little standard data about the vermiform appendix in Iranian population. Therefore, the objective of this study was to investigate the normal appendix size in Iranian cadavers. Materials and methods: A cross-sectional study was undertaken between June 2014 and July 2015, in the autopsy laboratory, Legal Medicine Organisation, Razavi Khorasan province, Iran. A total of 693 cadavers with the mean age of 40.46 ± 20.99 years were divided into 10 groups. After writing down position of the appendix, the length, diameter and weight of the appendix were measured. Statistical analysis was performed using SPSS software. Results: The mean values of the demographic characteristics included — age: 40.46 ± 20.99 years; weight: 63.47 ± 17.84 kg; height: 159.95 ± 28.23 cm. The mean values of the appendix length, diameter, weight and index in the cadavers were 8.52 ± 2.99 cm, 12.17 ± 4.53 mm, 6.43 ± 3.26 g and 0.013 ± 0.01, respectively. The most common position of the appendix was retrocaecal in 71.7% of cases. Significant correlations were evident between the value of demographic data and appendix size (p < 0.05). The diameter (p = 0.002) and index of the appendix (p = 0.003) showed significant difference between males and females. Conclusions: Having standard data on the vermiform appendix is useful for clinicians as well as anthropologists. The findings of the present study can provide information about morphologic variations of the appendix in Iranian population. (Folia Morphol 2017; 76, 4: 695–701)
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