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Laboratory experiments were conducted to determine the repellent activities of the ornamental plant Dioscorea sansibarensis leaf essential oil against the Bruchus chinensis bean weevil in the protection of stored legumes. The leaves exhibited potential repellency in a Y-tube olfactometer. The tabulated data show that 10μL of leaf oil exhibited a repellency of 41.33%, with a grouped median of 53.33%, which was better than 10 μL of Actellic 50 EC, which had a repellency of 36.00% and a grouped median of 40%. Statistically, there was no significant difference in the percentage repellency obtained from the leaf oil and Actellic 50 EC at different concentrations (10, 20, 30 and 40 μL/mL, X2(7, N=200) = 114.93, P< 0.05). The GC-MS analysis identified 16 compounds in the leaf oil. The main compounds and their percentage composition were phytol (19.46%), 1-epi-α-gurjunene (11.71%), palmitic acid (10.48%), ethyl palmitate (8.87%), methyl palmitate (7.72%), isophytol (5.99%), 2-heptadecanone (4.59%) and α-selinene (4.5%). The repellency caused by the by leaf oil may have been due to the presence of α-selinene, also known as naphthalene, 1,2,3,5,6,7,8,8a-octahydro-1,8a-dimethyl-7-(1-methylethenyl)-, [1S-(1.α.,7.α.,8a.α.)]-. Dioscorea sansibarensis may be a good choice for repellent formulations.
Dioscorea sansibarensis bulbils are well known for their toxicity and medicinal applications. In this study, the attraction and mortality of the Phaseolus vulgaris weevil (Bruchus chinensis) caused by Dioscorea sansibarensis bulbil oil was examined in a Completely Randomized Design experiment using a Y-tube olfactometer on a laboratory bench. Statistical analysis at P < 0.05 showed 10 μL had an attraction of 42.67%. In the case of mortality, the results showed that Phaseolus vulgaris seeds treated with 40 μL of the bulbil oil had a harmonic mean of 55.8% mortality of the weevil population, which was higher than with 10 μL of Actellic 50 EC. GC-MS analysis identified 17 compounds in the bulbil oil, the main constituents of which were 2-pentanone, 4-hydroxy-4-methyl- (17.8%), phenol, 4-ethyl- (11.41%), hexadecanoic acid, methyl ester (11.05%), phenol (7.35%), 9-octadecenoic acid, (E)- (7.2%) and phenol, 4-ethyl-2-methoxy- (7.14%). Attraction by bulbil oil may have been due to the presence of phenolic compounds, and mortality may have been due to hexadecanoic acid, methyl ester and 9-octadecenoic acid, (E)-. Attractants are used for the surveillance and detection of an infestation in stored products. The added advantage of the bulbil oil to cause mortality makes Dioscorea sansibarensis bulbil oil useful in the formulation of a botanical pesticide in stored pest management.
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