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The effects of proanthocyanidin on testicular toxicity in rats exposed to a glyphosate-based herbicide

100%

Avdatek F., Kirikkulak M., Yeni D.

Polish Journal of Veterinary Sciences

2023

tom 26

nr 4

The purpose of this study is to determine the effects of proanthocyanidin (PA) on spermatological parameters and testicular toxicity in male rats exposed to glyphosate (GLP). In our study, four groups were formed out of 24 male rats, each group would include 6 rats. The rats in the PA group were given a dose of 400 mg/kg/day dissolved in DMSO via gastric gavage. The rats in the GLP+PA groups were first given GLP at the LD50/10 dose of 787.85 mg/kg/day, followed by administering PA at a dose of 400 mg/kg/day dissolved in DMSO via gastric gavage. The rats in the GLP group were given GLP at the LD50/10 dose of 787.85 mg/kg/day dissolved in DMSO via gastric gavage. It was determined that in terms of motility, in comparison to the control group, the decreases in the GLP group and the increases in the PA and GLP+PA groups were statistically significant (p<0.001). The administration of GLP increased DNA damage compared to the control group, but the GLP+PA and PA applications reduced DNA damage (p<0.001). The analysis of testosterone levels indicated a statistically significant reduction in the GLP group compared to the other groups. Consequently, it was determined that PA effectively prevented the decreases in the spermatological parameters lowered as a result of GLP exposure and the oxidative stress and toxicity in testicular tissue.

The cryoprotective potential of propolis supplemented in frozen-thawed bull semen; biochemical and physiological findings

64%

Yeni D., Gulhan M. F., Inanc M. E., Avdatek F., Gungor S., Turkmen R., Tuncer P. B., Tasdemir U.

Polish Journal of Veterinary Sciences

2022

tom 25

nr 1

In this study, the cryoprotective effect of different doses of propolis (P) on bull semen, which has solid pharmacological properties thanks to its rich phenolic omponents, was investigated biochemically and physiologically. Semen samples were collected from Simmental breed bulls via the artificial vagina and pooled. After dividing into five groups, control (C: no additive) and four different P (200, 100, 50, and 25 μg/mL) groups, the final concentration was diluted to 16×106 per straw. Semen samples were equilibrated at 4°C for approximately 4 hours, then placed in French straws and frozen. After thawing, sperm motility and kinetic parameters, DNA integrity by single-cell gel electrophoresis, sperm abnormalities by liquid fixation, and lipid peroxidation levels by the colorimetric method was analyzed by Computer-Assisted Semen Analyzer. P added to the diluent showed no effect on motility and kinetic parameters at P25 and P50 (p>0.05), while P100 and P200 had a negative effect (p<0.001). The addition of P (25 and 50) showed a treatment effect on tail abnormality compared to C (p<0.05). Especially P50 had a positive effect on tail length, tail DNA, and tail movement, while P100 and P200 caused DNA damage (p<0.001). MDA levels increased in all P dose groups compared to C (p<0.001). This study has clearly demonstrated that P25 and P50 supplements could be used therapeutically to treat sperm tail abnormalities and prevent DNA damage in ost-thawed bull sperm.

Ograniczanie wyników

2 Polish Journal of Veterinary Sciences

2 Avdatek F.

2 Yeni D.

1 Gulhan M. F.

1 Gungor S.

1 Inanc M. E.

1 Kirikkulak M.

1 Tasdemir U.

1 Tuncer P. B.

1 Turkmen R.

1 2023

1 2022

Wyniki wyszukiwania

The effects of proanthocyanidin on testicular toxicity in rats exposed to a glyphosate-based herbicide

The cryoprotective potential of propolis supplemented in frozen-thawed bull semen; biochemical and physiological findings