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1

Review of the subgenus Paragreenidea Raychaudhuri of Greenidea Schouteden with description of one new species [Hemiptera: Aphididae: Greenideinae]

100%
Zhang D., Qiao G.
Annales Zoologici
|
2007
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tom 57
|
nr 3
The aphid subgenus Paragreenidea Raychaudhuri, 1956 is reviewed, and one new species, Greenidea (Paragreenidea) cayratiae Qiao et Zhang sp. nov. on Cayratia japonica from Fujian Province, China is described here. Keys to the four known species worldwide, and descriptions of apterous and alate viviparous females and four stages larvae of the new species are also given. Type specimens of the new species are deposited in the Zoological Museum, Institute of Zoology, Chinese Academy of Sciences, Beijing, China (ZMCAS).
2

Identyfication of differentially expressed genes preferably related to drought response in pigeon pea (Cajanus cajan) inoculated by arbuscular mycorrhizae fungi (AMF)

81%
Qiao G., Wen X., Yu L., Ji X.
Acta Physiologiae Plantarum
|
2012
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tom 34
|
nr 5
Pigeon pea is an ideal crop for sustainable agriculture systems in Karst areas of southwest China, which frequently suffers from the formidable water deficit. Physiologically, arbuscular mycorrhizae (AM)-colonized pigeon pea (Cajanus cajan) demonstrated a further enhanced tolerance to drought stress. To elucidate the molecular mechanism underlying the elevated tolerance, suppression subtractive hybridization (SSH) were employed to dig up the differentially expressed genes using mixed cDNAs prepared from drought-stressed and unstressed pigeon pea seedlings inoculated by AM fungi (AMF) in the present work. Both forward and reverse SSH cDNA library were constructed and a total of 768 clones were obtained. Dot-blotting expression analysis identified that 142 clones were upregulated, and 49 were downregulated during water stress. After sequencing, 182 unique expressed sequence tags (ESTs) were obtained via blast analysis, among which 142 (78%) exhibited high homology to previously identified or putative proteins, however, 40 (22%) showed no homology in the database. The upregulated (102) and downregulated (40) ESTs with significant protein homology might be sorted into 16 and 12 functional categories respectively, which involved in a broad spectrum of biological pathways. Furthermore, semi-quantitative reverse transcription (RT)-PCR was carried out for the 35 differentially expressed genes whose putative functions implicated in abiotic stress tolerances in other species, and it was verified these differentially expressed genes highly involved in drought stress tolerance of AM-colonized pigeon pea.
3

Review of genus Lithoaphis Takahashi, 1959 [Hemiptera: Aphididae: Hormaphidinae] from China, with the description of a new species

81%
Qiao G., Guo K., Zhang G.
Annales Zoologici
|
2005
|
tom 55
|
nr 3
Genus Lithoaphis Takahashi, 1959 is reported from China. One new species Lithoaphis quercisucta sp. nov. from Yunnan Province, China is described. Diagnosis character, key of species, distribution, host plant and biology of the genus Lithoaphis are provided. Morphological description, feature pictures, distribution, and host plant of the new species are included in this paper. All specimens including the type are deposited in the Zoological Museum, Institute of Zoology, Chinese Academy of Sciences, P. R. China.
4

Tuberolachnus [Tuberolachniella] macrotuberculatus sp. nov. from China [Hemiptera: Aphididae: Lachninae]

81%
Yang J., Qiao G., Zhang G.
Annales Zoologici
|
2005
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tom 55
|
nr 3
The aphid subgenus, Tuberolachnus (Tuberolachniella) Hille Ris Lambers et Basu, 1966 is newly recorded from China. A new species, Tuberolachnus (Tuberolachniella) macrotuberculatus sp. nov. is described. The types are deposited in Zoological Museum, Institute of Zoology, Chinese Academy of Sciences.
5

Transgenic poplar ‘‘NL895’’ expressing CpFATB gene shows enhanced tolerance to drought stress

61%
Zhang L., Liu M., Qiao G., Jiang J., Jiang Y., Zhuo R.
Acta Physiologiae Plantarum
|
2013
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tom 35
|
nr 02
Acyl-ACP thioesterases are responsible for the export of fatty acids produced by the de novo fatty acid synthesis system from the plastid. A fatty acyl–acyl carrier protein thioesterase (CpFATB) was isolated from a Chimonanthus praecox (linn.) Link. (wintersweet) cDNA library. This gene was subsequently transferred into Populus deltoides CL 9 P. euramericana CL ‘‘NL895’’ through Agrobacterium tumefaciens-mediated leaf disc transformation. The transgenic plants were confirmed by polymerase chain reaction (PCR) analysis and Southern hybridization. Three positive transgenic lines each with a single T-DNA insertion were obtained. Reverse transcription PCR (RT-PCR) confirmed CpFATB expression in transformed plants after short-term exposure to drought. When exposed to drought stress, transgenic plants showed higher superoxide dismutase (SOD) activity, peroxidase (POD) activity, catalase (CAT) activity, chlorophyll a content, chlorophyll b content and total chlorophyll content compared with untransformed plants. Malondialdehyde (MDA) content, electric conductivity and net photosynthetic rate of the transgenic plants were lower than untransgenic plants. The results of physiological indices showed that the overexpression of CpFATB in plants exhibited higher drought stress tolerance under drought stress condition compared to the untransformed control plants. Our data further confirmed and highlighted the functions of CpFATB in enhancing plants drought tolerance and may provide a plausible approach to breed plants combating drought stress and enlarging living scope.
6

Adventitious bud regeneration from leaf explants of Platanus occidentalis L. and genetic stability assessment

51%
Sun Y., Zhao Y., Wang X., Qiao G., Chen G., Yang Y., Zhou J., Jin L., Zhuo R.
Acta Physiologiae Plantarum
|
2009
|
tom 31
|
nr 1
The aim of this work is to develop a method of plant regeneration from leaf explants of Platanus occidentalis L. successfully. Woody plant medium (HortScience 16:453–459, 1981) and Murashige and Skoog (Physiol Plant 15:473–497, 1962) medium were used as induced and rooted basal medium, respectively. The effects of combinations of 6-BA, IBA, NAA and KT with different concentrations on adventitious bud regeneration from P. occidentalis leaf explants were compared. The results showed that the highest shoot regeneration frequency (90%) and maximum number (13.72 ± 0.44) of shoots per explant was recorded on WPM medium supplemented with 22.20 mmol l⁻¹ 6-BA and 0.49 mmol l⁻¹ IBA. A 40-day-old explants were much more productive for shoot formation than others in this study. The regenerated shoots were cultured on MS medium supplemented with 1.33 mmol l⁻¹ 6-BA, 0.16 mmol l⁻¹ NAA and 2% (w/v) adenine, after 2-week shoots were transferred to 1/2 MS medium supplemented with 0.49 mmol l⁻¹ IBA for rooting. Hardened plantlets via acclimatization were transferred to pots and transplanted to the soil finally. To ascertain whether tissue culture had effects on the genetic stability of plantlets regenerated, the genetic diversity was assessed using RAPD marker. A total of 96 bands ranging from 0.5 to 2.2 kb with an average of 6.4 bands per primer, were obtained using 15 primers. Amplified products exhibited few of polymorphic patterns across all the plants of P. occidentalis and the overall frequency of detection of somaclonal polymorphisms was lower than 0.0104%.
7

Program death-1 suppresses autoimmune arthritis by inhibiting Th17 response

51%
Yang L., Qiao G., Hassan Y., Li Z., Zhang X., Kong H., Zeng W., Yin F., Zhang J.
Archivum Immunologiae et Therapiae Experimentalis
|
2016
|
tom 64
|
nr 5
8

Over-expression of the Arabidopsis Na plus H plus antiporter gene in Populus deltoides CL x P. euramericana CL "NL895 "enhances its salt tolerance

51%
Qiao G., Zhuo R., Liu M., Jiang J., Li H., Qiu W., Pan L., Lin S., Zhang X., Sun Z.
Acta Physiologiae Plantarum
|
2011
|
tom 33
|
nr 3
Soil salinity is a serious problem worldwide. It is necessary to improve the salt tolerance of plants to avoid the progressive deterioration of saline soil. We showed that the over-expression of AtNHX1 improves salt tolerance in a transgenic poplar (Populus deltoides CL × P. euramericana CL ‘‘NL895’’) under mannose selection. Four transgenic poplar plants were obtained. Southern blot analysis showed that the pmi gene had integrated into the genome of the poplar. RT-PCR confirmed that AtNHX1 could be expressed normally in the transgenic plants. When tested for salt tolerance by NaCl stress, we measured a 100% increase in Na⁺ content in the three transgenic lines (T18, T50, T98) significantly higher than the 33% increase seen in wild-type plants. The chlorophyll content of the transgenic plants was not altered significantly, while the chlorophyll content in the control plants showed a small decrease. MDA content was decreased in the transgenic plants. These results show that the AtNHX1 gene may enhance salt tolerance due to increased vacuolar compartmentalization of sodium ions.
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