The total carbon and nitrogen content in soil, yield of plants, as well as uptake of N by plants were measured in the last 34 years of the long-term static experiments started in 1923 on the Experimental Field at Skierniewice which belongs to Department of Agricultural Chmistry, Warsaw Agricultural University. The total soil carbon and nitrogen were determined in 1995 in soils, which were sampled every 10 years since 1961 and stored at the laboratory in closed boxes in dry conditions. The total C and N conteent in soil were higher on the plots with manure than on plots with mineral fertilizers. On the contrary, on the control plots (CaPK treatment), the similar level of the elements has been formal facture last 34 years. Mineral fertilization resulted in higher yields and lower N-losses from soil than on plots with farmyard manure. The ratio of C:N in soil at the same treatments was stable for many years. The uptake of N by plants from the soil on CaPK treatment is about 30 kg/ha yearly.