Artificial spawning of tench collected from wild populations was studied under controlled conditions. In first part of present work, the accurate moment of applying hormonal injection was tested. The best results were obtained, when fish were stimulated between 1st and 3rd day after their collection. From tested hormonal combination (second part of work) ovopel containing GnRHa and dopamine antagonist: metoclopramide, produced the best results. All tested hormones influenced in high quality of oocytes. The differences were observed in the latency time. Fish stimulated by carp pituitary extract (CPE) ovulated much earlier than those stimulated by ovopel. Spawning techniques described in this paper were successful and provided consistent results in reproduction of tench collected from wild populations.