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Although information on the reproductive biology of the endangered plant family Gesneriaceae is well known, the pollination mechanisms of these plants in karst regions are poorly understood. This study demonstrated the flowering phenology, pollinators, and breeding system of Hemiboea ovalifolia in karst regions. Findings revealed that the anthesis of H. ovalifolia often occurred late, during sunset, or early morning, with duration of 2-4 days; there was a certain level of temporal overlap between pollen viability and stigma receptivity; the most effective pollinators were Bombus ignitus and Anthophora zonata. Controlled pollination indicated that these plants were pollen limited and exhibited late-acting inbreeding depression resulting from the seed sets; there were significant differences in fruit sets between open-pollination and self-pollination or cross pollination, and in seed sets between self-pollination and cross-pollination or open-pollination. Despite the co-existence of large numbers of fruit and seed sets, and vegetative propagation in H. ovalifolia, a failure in seedling survival, and long duration to establishing first-year seedlings in natural populations suggests that the species does not easily recover from damage.
Human activity has greatly increased the amount of biologically available nitrogen entering the natural environment. Addition of N can affect growth of and competitive interactions between native and invasive plants, thus increasing or decreasing the risk of invasion by alien species. Bidens frondosa is an invasive weed native to North America that recently has begun to spread in China. The influence of soil nutrient content on B. frondosa invasion has not yet been reported. In a common garden experiment, we compared the growth and competitive effects between B. frondosa and its co-occurring native congeners — B. tripartita and B. biternata — under three N levels (0 g m⁻², 1 g m⁻², 5 g m⁻²) to assess whether increased levels of N modifies risk of B. frondosa invasion. Our results showed that while N additions increased both the growth and competitive advantage of B. frondosa as compared with that of the native congener species, results are particularly pronounced under high N levels. While growth responses to N addition varied little among invasive populations under high N levels, the competitive effect of B. frondosa did vary among populations and was significantly greater than that of the congeners. Anthropogenic N additions are likely to increase risks of B. frondosa invasions. Thus, management efforts should focus on the reduction of N input to ecosystems to mitigate invasions by B. frondosa.
Prompt and accurate diagnosis is necessary to start adequate treatment for different affecting species including P. falciparum and P. vivax. Here we described the Wondfo Rapid diagnostic Kit (Pf-HRP2/PAN-pLDH) for the detection of P. falciparum and pan-plasmodium in patient specimen by using a nano-gold immunochromatographic assay. Our rapid assay adapted nano-gold labeling techniques and the monoclonal antibodies (mAbs) against both histidine rich protein-2 (Pf HRP-2) of P. falciparum and pan plasmodium-specific pLDH (pan pLDH). The established two-antibody sandwich immunochromatographic assay could detect P. falciparum and pan-plasmodium. The sensitivity and specificity of Wondfo rapid diagnostic kit were determined by comparing with the “gold standard” of microscopic examination of blood smears. In this study1023 blood samples were collected from outpatient clinics in China and Burma, and detected by both Wondfo kit and microscopic examination. The detection sensitivity and specificity of Wondfo rapid diagnostic kit were 96.46% and 99.67% for P. falciparum (HRP2), 95.03% and 99.24% for pLDH, 96.83% and 99.74% for non-falciparum species, 96.70% and 99.74% for P. vivax, respectively. These results indicate that Wondfo rapid diagnostic assay may be useful for detecting P. falciparum and non-P. falciparum (especially P.v.) in patient specimen.
An in vitro cumulative gas production technique was used to evaluate the nutritional characteristics of different botanical fractions of mature maize stover (variety Kexiang No.11). Whole maize stover was separated into eight fractions: leaf blade, leaf sheath, whole stem, upper stem, lower stem, cob, husk, and tassel. The results showed that there were significant (P<0.05) variations among different botanical fractions in in vitro gas parameters (e.g., the theoretical maximum volume of gas production, the rate constant of gas production, and fermentation lag time). Husk had the highest theoretical maximum gas production and rate constant, while leaf sheath had the longest lag time. The hemicellulose content was positively related (P<0.05) with cumulative gas production at 24, 36 and 48 h of incubation and with the theoretical maximum gas production. The hemicellulose-to-crude-protein ratio was positively related (P<0.05) to the rate constant of gas production. In vitro gas production showed a negative but not significant (P>0.05) relationship with acid detergent fibre (ADF) and neutral detergent soluble (NDS) contents of the morphological fractions.
Controlled irrigation and drainage (CID) has received considerable attention as a reliable management practice for improving water quality and water productivity in rice production. This study aimed to evaluate the effects of CID on water productivity, nitrogen, and phosphorus losses in paddy fields. Treatments include alternate wetting and drying (AWD; lower limit of irrigation to -200 mm and upper limit of ponding water depth after rainfall to 60 mm), CID-I (lower limit of irrigation to -200 mm and upper limit of ponding water depth after rainfall to 200 mm), and CID-II (lower limit of irrigation to -500 mm and upper limit of ponding water depth after rainfall to 200 mm). Results showed that CID reduced irrigation water without a significant impact on grain yields and increased the irrigation water productivity by 14.6-51.5% compared with AWD. However, the percolation of CID may be increased, especially in a wetting year. The application of CID-II by combining yield with irrigation water productivity could be suitable and beneficial to rice crops. The average total nitrogen (TN) and total phosphorus (TP) concentrations of CID presented similar values or were significantly increased relative to AWD, indicating that the significant decreases in nutrient loads under CID were primarily due to reductions in surface runoff rather than changes in concentration. Ammonium nitrogen (NH₄⁺-N) concentrations were clearly increased after fertilizer application in percolation water. Compared with AWD, the NH₄⁺-N, TN, and TP leaching losses of CID-I were increased. The nitrogen and phosphorus leaching losses of CID-II were significantly increased relative to AWD and CID-I because of high nutrient concentrations and severe preferential flow. Therefore, CID potentially increased nitrogen and phosphorus loading to groundwater when the lower limit irrigation was used. The results indicate that the suitable application of CID can save fresh water, reduce nutrient losses, and guarantee rice production.
Pitaya contains various types of polyphenols, flavonoid and vitamins which are beneficial for health and it is among the most important commercial tropical fruits worldwide. Endophytic bacteria might be beneficial for plant growth and yield. However, bacterial diversity in pitaya is poorly characterized. In this study, fruits of white and red pitayas from three different origins (Thailand, Vietnam and China) were chosen for endophytic bacteria diversity investigation by using Illumina HiSeq second-generation high-throughput sequencing technology. Large number of endophytic bacteria were detected and 22 phyla, 56 classes, 81 orders, 122 families and 159 genera were identified. Endophytic bacteria diversity was uneven among pitaya fruits from different origins and bacteria structure was different between white pitaya group and red pitaya group. Phylum Bacteroidetes, classes Bacteroidia and Coriobacteriia, orders Bacteroidales and Coriobacteriales, families Prevotellaceae, Bacteroidaceae, Ruminococcaceae, Paraprevotellaceae, Rikenellaceae, Alcaligenaceae and Coriobacteriaceae, genera Prevotella, Bacteroides, Roseburia, Faecalibacterium and Sutterella were statistically significant different species (P < 0.05) between white and red pitayas. These findings might be useful for growth improvement, fruit preservation and processing of different pitaya species from different origins.
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