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Alpha-galactosidase in immobilized cells of Citrullus vulgaris L.

100%

Stano J., Micieta K., Tokhtaeva E., Fulmekova M.

Herba Polonica

2005

tom 51

nr 3-4

Cells of suspension culture Citrullus vulgaris cv. "Samara" were permeabilized by Tween 80 and immobilized by glutaraldehyde. The highest a-galactosidase activity was at pH 5.4 and 60°C. The hydrolysis of substrate was linear for 3.5 h reaching 65-70% conversion of the substrate. The cells characterized with high enzyme activity, and stability in long-term storage showed convenient physico-mechanical properties (physical protection from shear forces and easy separation of product from biocatalysts).

Identification and determination of extracellular saccharase in cell suspension of Chelidonium majus L.

76%

Micieta K., Stano J., Korenova M., Blanarikova V., Fulmekova M., Matejka P.

Herba Polonica

2005

tom 51

nr 1-2

A simple and rapid procedure for identification and determination of extracellular saccha- rase is described, using a culture medium of Chelidonium majus L. (celandine) cell suspension cultures. Sucrose was used as a substrate for determination of the extracellular and intracellular activities of the studied enzyme. The culture medium (without cells) was used for identification and determination of extracellular enzyme activity. Intracellular activity was estimated using cell suspension.

Ograniczanie wyników

2 Herba Polonica

2 Fulmekova M.

2 Micieta K.

2 Stano J.

1 Blanarikova V.

1 Korenova M.

1 Matejka P.

1 Tokhtaeva E.

2 2005

Wyniki wyszukiwania

Alpha-galactosidase in immobilized cells of Citrullus vulgaris L.

Identification and determination of extracellular saccharase in cell suspension of Chelidonium majus L.