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1

Reakcja amplifikacji bakteryjnego DNA w diagnostyce infekcyjnego zapalenia wsierdzia

100%
Hryniewiecki T., Gzyl A., Augustynowicz E., Rawczynska-Englert I.
Medycyna Doświadczalna i Mikrobiologia
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2002
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tom 54
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nr 3
265-272
W pracy oceniono przydatność reakcji amplifikacji fragmentów bakteryjnego DNA w rozpoznawaniu infekcyjnego zapalenia wsierdzia o etiologii bakteryjnej oraz określono swoistość i czułość reakcji. Do amplifikacji wybranych fragmentów bakteryjnego DNA wykorzystywano swoiste startery F/R dla konserwatywnego regionu kodującego obszar 16S rRNA Procaryota. Wykazano, że świeża krew oraz surowica są lepszymi w stosunku do krwi zamrażanej (-20°C) materiałami do badania. Uzyskane wyniki wskazują, że zastosowana metoda PCR jest przydatna do identyfikacji bakteryjnych czynników zakażenia.
2

Pericardium: structure and function in health and disease

100%
Jaworska-Wilczynska M., Trzaskoma P., Szczepankiewicz A. A., Hryniewiecki T.
Folia Histochemica et Cytobiologica
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2016
|
tom 54
|
nr 3
3

Kliniczne znaczenie modyfikowanej niedokrwieniem albuminy u pacjentów z zawałem mięśnia serca i prawidłowym stężeniem troponiny I

100%
Gozdzik D., Hryniewiecki T., Sitkiewicz D.
Diagnostyka Laboratoryjna
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2007
|
tom 43
|
nr 3
4

In vivo effect of 2-deoxy-D-glucose on adenine nucleotide levels in the liver and skeletal muscle of rats

100%
Torlinska T., Rutkowska D., Hryniewiecki T., Paluszak J.
Acta Physiologica Polonica
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1990
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tom 41
|
nr 7
Torlińska T., Rutkowska D., Hryniewiecki T., Paluszak J.: In vivo effect of 2-deoxy-D-glucose on adenine nucleotide levels in the liver and skeletal muscle. Acta Physiol. Pol. 1990, 41 (7): 75-83. The present report indicates that 2-deoxy-D-glucose (2-DG) at a single dose causing reduction of Т„ has no influence on liver and skeletal muscle content of ATP, ADP and AMP, the ATP/ADP ratio, energy charge potential (ECP) and total adenine nucleotides (TAN). After administration of 2-DG for 3) successive days, the level of ATP, ATP/ADP ratio, the values of ECP and TAN are decreased both in the liver nad skeletal muscle. However, 72 hours after the last injection of 2-DG adenine nucleotide contents returned to the values observed in control group, indicating that the in vivo effect of this glucose analogue is fully reversible.
5

In vivo effect of 2-deoxy-D-glucose on glucose-6-phosphate dehydrogenase activity in the cytosol of liver, heart and skeletal muscle of rats

100%
Torlinska T., Ozegowski S., Paluszak J., Hryniewiecki T.
Acta Physiologica Polonica
|
1990
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tom 41
|
nr 7
Torlińska T., Ożegowski S., Paluszak J., and Hryniewiecki T.: In vivo effect of 2-deoxy-D-glucose on glucose-6-phosphate dehydrogenase activity in the cytosol of liver, heart and skeletal muscle of rats. Acta Physiol. Pol. 1990, 41 (6): 137-143. 2-deoxy-D-glucose (2-DG), the unmetabolizable analogue of glucose induces a series of metabolic, hormonal and behavioral responses, causing cellular glucoprivation. According to in vitro studies, 2-DG inhibits phosphofructokinase in cultured human cells. The present investigations deal with changes in the cytosolic glucocse-6-phosphate dehydrogenase activity following in vivo 2-DG administration. A single dose of 2-DG (600 mg/kg) has no influence on the activity of glucose-6-phosphate dehydrogenase in the cytosol of liver, heart and skeletal muscle of the rat. The concommitant increase in serum glucose, lactate and FFA concentrations observed in the study indicates indirectly a stimulation of adrenergic system. After three days of successive administration of 2-DG to rats, dehydrogenase activity decreased in the liver by approx 57% and in the skeletal muscle by approx 82% in comparison with control animals. Moreover the in vivo effect of 2-DG was found to be fully reversible, probably when the total amount of the inhibitor was excreted.
6

High fat content in diets and oxidative stress in livers of non-diabetic and diabetic rats

88%
Marczuk-Krynicka D., Hryniewiecki T., Paluszak J., Krauss H., Nowak D.
Polish Journal of Environmental Studies
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2009
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tom 18
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nr 2
249-253
Environmental factors such as high fat content in a diet affect pro- and antioxidative balances in tissues. Our study was designed to determine whether a four-week diet enriched to a total of 15% fat content with either a polyunsaturated (linoleic) or saturated (palmitic) fatty acid predisposes or protects the liver tissue against oxidative stress in both non-diabetic and diabetic rats. In the rat liver the activity of catalase, superoxide dismutase, glutathione peroxidase, and the level of thiobarbituric acid reactive substances were determined. Our study suggests that both diets induce oxidative stress in livers of non-diabetic rats. However, in diabetic rats a diet enriched in linoleic acid appears to attenuate oxidative stress.
7

A levels of endogenous gonadal hormones and their relationship with selected coronary artery disease risk factors among young women post myocardial infarction

88%
Ablewska U., Jankowski K., Rzewuska E., Liszewska-Pfejfer D., Hryniewiecki T.
Acta Biochimica Polonica
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2011
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tom 58
|
nr 3
In recent decades a significant raise in the incidence of myocardial infarction among young women has been recorded. It is presumed that, apart from the classical risk factors, other reasons exist for premature atherosclerosis in young women, related to the homeostasis of gonadal hormones. The aim of the study was to analyze the levels of gonadal hormones (estradiol, progesterone, follicle-stimulating hormone, luteinizing hormone, testosterone and dehydroepiandrosterone) measured in the luteal phase, in 65 normally menstruating women post myocardial infarction (MI) and to investigate a possible relationship between the hormone profile and selected coronary artery disease (CAD) risk factors. The levels of gonadal hormones: estradiol, progesterone, follicle-stimulating hormone, luteinizing hormone, testosterone and dehydroepiandrosterone were measured in the luteal phase. All examined women had normal mean levels of gonadal hormones. In the post MI patients leading a sedentary life style, a significantly lower mean progesterone concentration was observed (16.29 ± 9.11 versus 29.43 ± 21.14 nmol/l, p = 0.05) and significantly higher mean testosterone concentration (2.34 ± 0.98 versus 1.76 ± 1.09 nmol/l, p = 0.05) when compared to patients from the same group, but leading a more active life. In obese post MI women (BMI ≥ 30 kg/m2) a lower mean concentration of progesterone was detected (18.02 ± 8.12 versus 26.16 ± 14.72 nmol/l, p = 0.05), than in slimmer patients from the same group. In post MI women with a positive family history for CAD, a significantly higher mean concentration of testosterone was detected (2.31 ± 1.22 versus 1.67 ± 0.74 nmol/l, p = 0.05) than in patients with no family history. The results suggest a correlation between levels of gonadal hormones and classical CAD risk factors.
8
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Age dependent changes of insulin receptors in rat tissues

64%
Torlinska T., Mackowiak P., Nogowski L., Hryniewiecki T., Witmanowski H., Perz M., Madry E., Nowak K. M.
Journal of Physiology and Pharmacology
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2000
|
tom 51
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nr 4,2
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