The cultivation of Asp. awamori strain NRRL 3112 was carried out in deep culture on a shaker in Erlenmayer flasks during 120 h. Six types of starch were investigated as the carbon source combined with mineral and organic nitrogen. The potato mash or wheat as complete C and N sources, were also investigated. Glucoamylase (GA) and α-amylase activities, glucose contents the dynamics of pH changes and the starch saccharification ability (DE) of glucoamylase were determined. All starch types investigated were shown to be the effective C sources for the high level of GA synthesis and the best N source was found to be wheat bran. The most effective nutrient as concerns the GA synthesis was that containing potato pulp and wheat bran.
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