Nosemosis is a serious honeybee disease linked to Colony Collapse Disorder (CCD). It cause many changes at the individual bee level, which also affects the health of the entire bee colony. N. ceranae and N. apis are not tissue specific as was previously thought and besides the ventriculus epithelium their spore are also present in other tissue, such as Malpighian tubules, hypopharyngeal glands, salivary glands, and fat bodies. Emplacement of nosema infection in honeybee glands interferes with the production of the royal jelly, honey, bee bread. Moreover spores remaining in the honeybee glands are a potential reservoir of infection. The aim of the research was to determine the correlation among the number of Nosema spores in whole bees, as well as in their ventriculus and hypopharyngeal glands. Nosema-infected honey bees were collected in the spring, when there should be a comparable degree of Nosema infection level in all tissues. Three independent experiments were conducted. In these studies the number of spores in the hypopharyngeal glands was the lowest and the highest results were observed for ventriculus samples. A large number of spores in the hypopharyngeal glands was also observed. This can be the cause of a reduction or loss of these glands’ function; moreover, it may increase the horizontal transmission of the infection within a hive as well as to a queen bee.