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1

Effects of carbon source, polyethylene glycol and abscisic acid on secondary embryo induction and maturation in rapeseed (Brassica napus L.) microspore-derived embryos

100%
Yadollahi A., Abdollahi M. R., Moieni A., Danaee M.
Acta Physiologiae Plantarum
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2011
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tom 33
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nr 5
In this study, the effects of carbon sources, abscisic acid (ABA) either alone or in combination with polyethylene glycol (PEG) were evaluated on secondary embryo (SE) induction and maturation in rapeseed microspore-derived embryos (MDE) of cultivars Global, PF704 and Option. Among various carbon sources tested (sucrose, glucose, fructose and sorbitol), the use of 0.3 M (300 mOsml⁻¹) glucose and 0.2 M (200 mOsml⁻¹) sorbitol in SE induction medium (for cultivars Global and PF704) and sorbitol at 0.2 and 0.3 M (200 and 300 mOsml⁻¹, for cultivar Option), induced the highest secondary embryogenesis percentage (%SE). The highest number of SEs per each MDE (SE/MDE) was observed with 0.2 M (200mOsml⁻¹) sorbitol in cultivar Global and with 0.3 M (300 mOsml⁻¹) glucose in cultivars PF704 and Option. In another part of this study, the effect of different concentrations of ABA (0, 20, 40, 60, 80 and 100 µM) and of a combined use of ABA (0 and 40 µM) and PEG 4000 or PEG 6000 at 15 g l⁻¹ (3.75 and 2.5 mOsml⁻¹, respectively) was examined on induction and maturation of SEs. In the first experiment, the use of ABA in SE induction medium reduced the mean number of SE/MDE in the three studied cultivars, whereas use of 40–80 µM ABA in SE induction medium increased the percentage of mature SEs in each cultivar. The combined use of PEG with or without ABA also reduced the mean number of SE/MDE compared with control, but resulted in significant enhancement of the percentages of mature SEs for the three cultivars.
2
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The mRNA sequence polymorphisms of flowering key genes in bolting sensitive or tolerant sugar beet genotypes

88%
Alimirzaee M., Mirzaie-Asl A., Abdollahi M. R., Kolaei H. E., Fasahat P.
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
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2017
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tom 98
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nr 3
3

An efficient method for transformation of pre-androgenic, isolated Brassica napus microspores involving microprojectile bombardment and Agrobacterium-mediated transformation

76%
Abdollahi M. R., Corral-Martinez P., Mousavi A., Salmanian A. H., Moieni A., Segui-Martinez P.
Acta Physiologiae Plantarum
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2009
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tom 31
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nr 6
The physical barrier imposed by the thick microspore wall constitutes an obstacle for an efficient Agrobacterium-mediated transformation of vacuolate microspores prior to androgenic induction and haploid embryogenic commitment. It is thus necessary to implement additional methods to overcome this drawback. In this study, we focused on the optimization of a protocol to allow for the exogenous DNA to enter the microspore in an efficient manner. We tested different options, based on microprojectile bombardment, to be applied prior to agroinfiltration. From them, the best results were obtained through co-transformation by microspore bombardment with DNA-coated microprojectile particles, followed by Agrobacterium tumefaciens infection. This method provides an efficient means to integrate extraneous DNA into rapeseed microspores prior to androgenesis induction.
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