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Interactions between Marek’s disease virus Rispens/CVI988 vaccine strain and adenovirus field strain in chicken embryo fibroblast (CEF) cultures

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Nieczyporuk J. S., Wozniakowski G., Samorek-Salamonowicz E., Czekaj H.

Polish Journal of Veterinary Sciences

2014

tom 17

nr 1

The aim of the study was to determine the influence of adenovirus infection on the replication of Marek’s disease virus vaccine strain Rispens/CVI988 during in vitro co-infection studies. Adenovirus field strain JN-5/10j was isolated from sick chickens. The study was conducted in chicken embryo fibroblast cultures (CEF). Monolayers of CEFs were infected with Rispens strain and field adenovirus strain JN-5/10j with different doses (101.0-103.0 TCID₅₀) in the following manner: a) simultaneously, b) first, infection with Rispens strain and after 24 h infection with adenovirus strain JN-5/10j and c) infection with adenovirus strain JN-5/10j 24 h before infection with Rispens strain. After 18, 24, 48, 72, and 96 h of incubation, the copy number of the pp38 gene of Rispens strain was determined using Real-time PCR. The results indicated that the Adenovirus infection before the infection with Rispens strain reduced the replication of the pp38 gene after 48 h by 2 log₁₀.

Ograniczanie wyników

1 Polish Journal of Veterinary Sciences

1 Czekaj H.

1 Nieczyporuk J. S.

1 Samorek-Salamonowicz E.

1 Wozniakowski G.

1 2014

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Interactions between Marek’s disease virus Rispens/CVI988 vaccine strain and adenovirus field strain in chicken embryo fibroblast (CEF) cultures