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Our survey of data collected in the Chromosome Number Database for Polish angiosperms indicated that the 1,498 species with chromosome counts represent 40% of the total angiosperms (3,719) occurring in Poland, including 1,205 native species (53% of native species) and 194 anthropophytes (56% of anthropophytes). The chromosome numbers are known for all native species occurring in Poland within 298 genera and 46 families, and for all anthropophytes from 79 genera and 11 families. The remaining angiosperm groups are less explored: chromosome counts from Poland are known for 9% of cultivated species and 5% of ephemerophytes. According to generic basic chromosome numbers, 46.44% of Polish angiosperms have been classified as polyploid. By three different threshold methods, the contribution of polyploid plants to the Polish flora is 64.64%, 50.89% or 42.89%. Polyploidy is more common among indigenous than non-indigenous plants, and the ploidy distribution among plants from the Polish Tatras does not differ significantly from that observed in the rest of native Polish plants.
We analysed chromosome lengths, karyotype structure, and nuclear DNA content (flow cytometry) in diploid (2n=20) and triploid (2n=30) European H. lupulus var. lupulus, American H. lupulus var. neomexicanus (2n=20) and Japanese ornamental hop, H. japonicus (F/2n=16; M/2n=17). Diploid female representatives of H. lupulus var. lupulus and H. l. var. neomexicanus differed in total length of the basal chromosome set (23.16 µm and 25.99 µm, respectively) and nuclear 2C DNA amount (5.598 pg and 6.064 pg) but showed similar karyotype structure. No deviation from the additivity, both in chromosome length and 2C DNA amount was evidenced in triploid monoecious H. lupulus (2n=30, XXY). H. japonicus showed different karyotype structure, smaller basal chromosome set (F/18.04 µm, M/20.66 µm) and lower nuclear DNA amount (F/3.208 pg and M/3.522 pg). There are first evaluations of nuclear genome size in diploid, not commercial representative of European H. lupulus var. lupulus and American H. lupulus var. neomexicanus and first attempt to determine the absolute male and female genome size in two Humulus species.
The male-specific DNA markers are very useful in molecular sexing of non-flowering plants and seeds of dioecious species. In this paper we identified ten Y chromosome-specific RAPD primers suitable for identification of male plants in three Cannabaceae species with sex chromosomes (Humulus lupulus, XX/XY; H. japonicus, XX/ XY1Y2; Cannabis sativa, XX/XY). Basing on the nucleotide sequence of the OPJ-09 RAPD product we developed the HJY09 SCAR marker, which is very efficient in sexing of Japanese hop.
Chromosome numbers are given for the following species of Taraxacum sect. Palustria from Poland: T. paucilobum Hudziok (2n = 24, 25), T. belorussicum Val. N. Tikhom. (2n = 24), T. subdolum Kirschner & Štěpánek (2n = 24), T. udum Jordan (2n = 24), T. trilobifolium Hudziok (2n = 24), T. bavaricum Soest (2n = 24), T. portentosum Kirschner & Štěpánek (2n = 32), T. vindobonense Soest (2n = 32), and T. brandenburgicum Hudziok (2n = 32). The chromosome numbers of T. belorussicum and T. portentosum are published for the first time, and for T. subdolum, T. bavaricum and T. brandenburgicum for the first time from Poland. The analyzed group of taxa is heterogenous in respect of stomatal size, and after pooling of data the tetraploids show bigger stomata than the triploids.
The morphology, karyotype structure and nuclear DNA amount of European and American representatives of alpine cat's-tail Phleum commutatum Gaud. were analysed. The evolutionary relationships within this group of taxa are discussed. It was shown that the American and European tetraploids are both morphologically and karyologically similar to each other and to the diploid race of this species from the Polish Carpathians. Diploid relatives of tetraploid P. commutatum i.e., P. commutatum (2x) and P. rhaeticum (Humphries) Rauschert, showed intra- and interspecific differences in nuclear 2C DNA values, whereas tetraploids (of both European and American origin) showed highly uniform nuclear DNA amounts. The 2C DNA uniformity and the greater mean size of the basal chromosome set in tetraploid P. commutatum as compared with its diploid relatives suggest that the change in genome size occurred during early evolution of the tetraploid race of P. commutatum, before its migration to America.
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