Wyniki wyszukiwania

1

Szkolne Ostoje Przyrody

100%

Gawlak M.

Salamandra:Magazyn Przyrodniczy

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2013

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nr 2

2

Zanieczyszczenia biologiczne jako problem bezpieczeństwa żywności

72%

Nawrot J., Gawlak M.

Progress in Plant Protection

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2009

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tom 49

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nr 1

3

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Owoc wawrzynka (Daphne L., Thymelaeaceae) - pestkowiec czy jagoda?

58%

Zielinski J., Guzicka M., Gawlak M.

Rocznik Polskiego Towarzystwa Dendrologicznego

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2017

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tom 65

4

Wplyw nadekspresji ferrytyny w skielkowanych ziarniakach pszenicy zwyczajnej na tempo rozwoju wolka zbozowego [Sitophilus granarius L.]

58%

Zielinska-Dawidziak M., Gawlak M., Warchalewski J. R.

Progress in Plant Protection

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2007

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tom 47

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nr 4

262-266

5

Noradrenaline modulates the membrane potential in medial prefrontal cortex pyramidal neurons via β1-adrenergic receptors and HCN channels

58%

Grzelka K., Gawlak M., Szulczyk P.

Acta Neurobiologiae Experimentalis

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2018

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tom 78

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nr Suppl.1

The noradrenergic system is essential in medial pre‑ frontal cortex (mPFC) physiology: noradrenaline (NA) acting via adrenergic receptors (α1, α2 and β) plays a sig‑ nificant role in the regulation of cognitive brain functions and affective processes. Impaired modulation of the mPFC by NA has been implicated in many neuropsychiatric dis‑ eases, e.g. posttraumatic stress disorder, attention deficit hyperactivity disorder, and depression. While the pres‑ ence of all adrenergic receptor subtypes has been report‑ ed in the mPFC, little is known regarding the mechanisms by which NA modulates mPFC neurons. The aim of this study was to investigate which adrenergic receptor sub‑ type controls the resting membrane potential and hold‑ ing currents in mPFC neurons. Secondly, we wanted to de‑ fine the cellular effector(s) and signaling pathway(s) in‑ volved in the action of NA. To answer these questions, we recorded the membrane potential and holding current us‑ ing patch‑clamp techniques. Gramicidin perforated‑patch and classical whole‑cell recordings were obtained from layer V mPFC pyramidal neurons in slices isolated from young rats. Distribution of the adrenergic receptor sub‑ types in mPFC was visualized with fluorescent immuno‑ histochemistry. NA evoked depolarization and inward cur‑ rent in the tested cells. Stimulation of α1‑ and α2‑receptors failed to evoke similar effects. Meanwhile, the nonselective β‑receptor agonist, as well as the selective β1‑receptor ag‑ onist, mimicked the effect of NA on holding current. The NA‑dependent inward current was considerably reduced by the selective β1‑receptor antagonist. The effect of NA was also attenuated, though to a smaller degree, by the se‑ lective β3‑receptor antagonist. At the same time, applica‑ tion of two different selective β3‑agonists evoked inward currentsin the tested neurons. Expression of β1‑ and β3‑re‑ ceptors in mPFC was confirmed with confocal microscopy. The β1‑related inward current was greatly decreased in the presence of Cs+ ions and ZD7288, a selective blocker of HCN (hyperpolarization‑activated cyclic nucleotide–gated) channels. It was not affected by selective blockers of dif‑ ferent signaling pathways known to be responsible for me‑ diating the effects from G‑protein‑coupled receptors (e.g. adenylyl cyclase‑PKA and phospholipase C‑PKC). However, it was significantly diminished by blockers of the βγ sub‑ unit‑dependent transduction system. We conclude that NA modulates the membrane potential and holding current of mPFC pyramidal neurons preferentially via β1‑receptors. The effects occur due to HCN channel activation and are probably mediated in a membrane delimited fashion by a βγ subunit released from the G‑protein. Stimulation of β3‑receptors may be partially responsible for the NA‑relat‑ ed effects. Supported by National Science Centre, Poland, grant 2014/15/N/NZ4/04760 and FW5/PM2/16.

6

Applicability of zymograms for detection of granary weevil (Sitophilus granarius L.) alfa-amylases left on grain after insects infestation

58%

Piasecka-Kwiatkowska D., Nawrot J., Gawlak M., Zielinska-Dawidziak M.

Progress in Plant Protection

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2013

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tom 53

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nr 1

50 wheat grains of Korweta variety were infested with ten or twenty males of granary weevil and exposed for five or ten days. After that time the beetles were removed, and the extracts from grains were prepared and zymograms were done. Clearing zones observed on zymograms, showed the presence of granary weevil α-amylase in all grain extracts obtained after insects feeding. The obtained revealed that both, hidden grain infestation by the pest, as well as grain contamination with the pest metabolism products were possible to detect using the native electrophoresis with zymograms. Although the intensity of clearing zones was larger when the extracts were obtained from the grain with a greater number of insects feeding for a long time, the relationship between the number of insects, time feeding and α-amylase activity was not clearly shown. The amount of granary weevil α-amylases left on grain by feeding males was related to the time of feeding as well as individual activity of enzyme secreted by the salivary gland.

7

Gelatinolytic activity is present in the interchromatin space of the neuronal cell nucleus

58%

Gawlak M., Gorkiewicz T., Wilczynski G.

Acta Neurobiologiae Experimentalis

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2009

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tom 69

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nr 3

Matrix metalloproteinases (MMPs) form an enzyme family which, by mainstream research, is implicated in extracellular matrix processing in physiological and pathophysiological conditions. Some of these proteins termed gelatinases, in particular MMP-2 and MMP-9, cleave gelatin as an artifi cial substrate. Surprisingly, a number of studies have revealed the presence of gelatinolytic activity in the cell nucleus. Although the phenomenon appears not to be artifactual, neither the identity nor the role of nuclear gelatinases has been established unequivocally. In the nervous system, nuclear gelatinolysis is detectable in normal conditions, yet it is induced by seizures, and stroke. We studied nuclear gelatinolytic activity by high resolution in situ zymography (ISZ) in sections of alcohol-fi xed, polyester wax-embedded normal rat brain. Ubiquitously distributed among the major brain areas the ISZ signal was present mainly in neurons. At high magnifi cation, our study revealed previously unrecognized mesh-like pattern of nuclear gelatinolytic which, by counterstaing with fl uorescent DNA-binding dye, represents an interchromatin space. The ISZ signal colocalized with the ribonucleoprotein compartment enriched in splicing components, identifi ed using an immunoreactivity of spliceosome assembly factor SC-35. This suggesting a function for MMPs in processes of gene-expression and/or RNA-processing and hypothetically involvement in remodeling of chromosome territories.

8

Kłopotliwe derenie - Cornus alba L. i C. sericea L. (Cornaceae). Dwa gatunki czy jeden?

58%

Zielinski J., Tomaszewski T., Gawlak M., Orlova L.

Rocznik Polskiego Towarzystwa Dendrologicznego

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2014

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tom 62

The history of taxonomy and problems with identification of two very similar and closely related dogwoods Cornus alba and C. sericea are discussed. They were described by Linnaeus on the basis of flowering cultivated specimens of unknown origin. When characterizing their fruits, Linnaeus got the information from the older, “pre-Linnean”, literature and wrote that they were white in C. alba (Linné 1767) and black in C. sericea (Linné 1771). It was soon pointed out that both taxa had white fruits, however, their specific identity has not been questioned for a long time. Cornus alba and C. sericea are considered to be geographically isolated – the former is recorded from Siberia and NE Europe, while the latter from North America, but both dogwoods are often cultivated and naturalize in many places outside their natural ranges. In European dendrological literature, in which both plants are usually mentioned, there are permanent controversies concerning the differences between them. Attempts are still made to distinguish them, mainly on the basis of their stones and leaf shapes. Narrow stones and abruptly narrowed leaf apex have been attributed to C. alba, while broad stones have been said to be characteristic to C. sericea. Our analysis reveals that shapes of stones of discussed taxa are very variable and their ranges of variability overlap to a considerable extent. The similar kind of variability can be observed in the shape of leaves of both dogwoods. In C. alba leaf blades are most often broadly elliptic and abruptly narrowed, while in C. sericea they are most often broadly ovate and gradually narrowed at the apex. It must be said that there are also numerous exceptions to the above scheme. The filigree pattern of cuticle and the wax crystals on the abaxial leaf surface are sometimes useful for distinguishing C. alba and C. sericea. Unfortunately both features are also variable as those characterized above. Taking into consideration the great similarity of discussed dogwoods and difficulties with their identification, in our opinion the broad species concept of C. alba (including C. sericea) is most reliable and practical. However, as it appears from presented results, both taxa are not fully identical, so the rank of subspecies proposed by Wangerin (1910) – C. alba L. subsp. alba and C. alba subsp. stolonifera (Michx.) Wangerin, seems to be most appropriate in their case. It facilitates identification of wild plants of C. alba s.l. both in flowers and fruit as well as in the vegetative state. It also helps avoid controversy with the classification of cultivars of uncertain origin.

9

Morphological characters of copulatory organs and eggs of Arion rufus and Arion lusitanicus

58%

Jaskulska M., Gawlak M., Kozlowski J.

Folia Malacologica

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2013

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tom 21

|

nr 3

10

Macrobiotus naskreckii sp. nov., a new tardigrade (Eutardigrada: Macrobiotidae) of the hufelandi group from Mozambique

58%

Bakowski M., Roszkowska M., Gawlak M., Kaczmarek L.

Annales Zoologici

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2016

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tom 66

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nr 2

In this paper, Macrobiotus naskreckii sp. nov., a new species of the hufelandi group from the Gorongosa National Park in Mozambique, is described. The analysis have revealed that M. naskreckii sp. nov. is most similar to if. kristenseni, M. ramoli and M. serratus. The new species differs from M. kristenseni mainly through a different shape of egg processes and clearly dentate lunules IV; from M. ramoli mainly by smaller eggs and a smaller number of egg processes on the circumference; from M. serratus mainly by the absence of the reticulation between the egg processes. This is a third record of tardigrades from Mozambique and apart of the new species reported here, three other taxa from Gorongosa National Park are provided: Minibiotus cf. intermedins, Minibiotus sp. and Paramacrobiotus cf. richtersi.

11

The effect of copper sulphate on the alimentary tract of Arion vulgaris Moquin-Tandon, 1855

58%

Gabala E., Gawlak M., Kaluski T.

Folia Malacologica

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2016

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tom 24

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nr 1

12

Muscarnic receptor regulation of pyramidal neuron membrane potential in the medial prefrontal cortex (mPFC)

58%

Kurowski, Maciej Gawlak, Paweł Szulczyk P. N., Gawlak M., Szulczyk P.

Acta Neurobiologiae Experimentalis

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2015

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tom 75

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nr Supl.

BACKGROUND AND AIMS: Damage to the cholinergic input to the prefrontal cortex has been implicated in neuropsychiatric disorders. Cholinergic endings release acetylcholine, which activates nicotinic and/or muscarinic receptors and regulates the membrane potential in medial prefrontal cortex (mPFC) neurons. The aim of this study was to clarify the mechanism responsible for control of the medial prefrontal cortex (mPFC) pyramidal neurons by muscarinic receptors. MATERIAL AND METHODS: Experiments were performed on mPFC pyramidal neurons in slices isolated from young (18–22- day-old) male rats. Recordings of membrane potential were performed with the gramicidin perforated-patch method in the absence of Ca2+ ions and in the presence of tetrodotoxin (TTX, 1 μM) in extracellular solution. RESULTS: Cholinergic receptor stimulation by carbamoylcholine chloride (CCh; 100 μM) evoked depolarization (10.0±1.3 mV), which was blocked by the M1/M4 (pirenzepine dihydrochloride, 2 μM) and M1 (VU 0255035, 5 μM) muscarinic receptor antagonists and was not affected by a nicotinic receptor antagonist (mecamylamine hydrochloride, 10 μM). CCh-dependent depolarization was greatly attenuated in the presence of an inhibitor of the βγ-subunitdependent transduction system (gallein, 20 μM). mPFC pyramidal neurons express Nav1.9 channels. CCh-dependent depolarization was abolished in the presence of antibodies against Nav1.9 channels in the intracellular solution and augmented by ProTx-I toxin (100 nM) in the extracellular solution. CONCLUSION: Activation of M1 muscarinic receptors evokes depolarization of mPFC pyramidal neurons due to activation of Nav 1.9-like Na+ channels via G-protein βγ-subunits (in a membranedelimited mode). The study was supported by NCN grants no: NN401584638, NN301572940 and WUM grant no: FW5/PM31D/14.

13

Using scanning electron microscopy to study slug morphology

58%

Kaluski T., Gawlak M., Cieluch P., Kozlowski J.

Folia Malacologica

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2012

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tom 20

|

nr 1

14

Ultrastructure of Hepatopancreas in Arion vulgaris and Arion rufus

58%

Gabala E., Gawlak M., Kaluski T.

Folia Malacologica

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2015

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tom 23

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nr 1

15

Wpływ czasu żerowania chrząszczy wołka zbożowego (Sitophilus granarius L.) na ziarnie pszenicy na masę ziaren i zawartość wyekstrahowanego białka

58%

Piasecka-Kwiatkowska D., Zielinska-Dawidziak M., Nawrot J., Gawlak M.

Progress in Plant Protection

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2011

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tom 51

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nr 2

Granary weevils feed on wheat grain causing huge losses of stored material. Experiment was carried out on wheat grain Korweta variety. Weighted on analytical balance 50 kernels were infested by 5 pairs of granary weevil adults. The insects were feeding 1, 2, 3, 5, 10, 20 and 30 days and after that were removed and kernels were weighted once again. Then the grains were grounded and proteins extracted with 0.1 M acetic buffer pH = 5. Protein content was determined by Bradford method. All each time of infestation samples were repeated five times. It was found that the grain weevil beetles feeding on the wheat grain, at least five days caused statistically significant losses in grain weight and extractable protein content.

16

Anatomical study on the developing pericarp of selected Rosa species (Rosaceae)

58%

Guzicka M., Zielinski J., Tomaszewski D., Gawlak M.

Dendrobiology

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2012

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tom 68

Results of anatomical studies on the developing pericarp of selected wild roses are presented. Using SEM and CLSM, the changes in the pericarp structure of 5 species have been observed during its formation, from the flowering stage to fully ripe achenes. In the morphological development of the pericarp of Rosa species two main phases can be distinguished: the phase of intensive growth of the pericarp during which the fruit achieves its final shape and volume, and the subsequent phase of pericarp ripening when no significant morphological changes in the pericarp occur. Similarly, in the process of the anatomical development of the pericarp two phases are noticeable, however, during both stages, great internal changes proceed in the fruit. The first phase consists of intensive cell divisions and enlargement, gradual thickening of cell walls and formation of all pericarp layers. Due to these changes, the pericarp achieves its final anatomical structure. The second phase, involving the pericarp ripening, is manifested in the modification of cell walls, mainly by their quick thickening, but first of all by their lignification. The lignification of pericarp cell walls begins in the inner endocarp; it proceeds in the outer endocarp, later in mesocarp and finishes in the hypodermal cells of the exocarp. The epidermal cells remain alive the longest and their walls do not (or hardly) become lignified. The death of all cells finishes the pericarp ripening.

17

Outsourcing uslug IT - ekonomia, efektywnosc, nowoczesnosc...

58%

Lisowska I., Gawlak M.

Przegląd Komunalny

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2003

|

nr 09

64-65

18

Zastosowanie metody immunochemicznej do oznaczania skazenia ziarna zboz przez wolka zbozowego [Sitophilus granarius L.]

51%

Piasecka-Kwiatkowska D., Rosinski M., Warchalewski J. R., Gawlak M., Nawrot J.

Progress in Plant Protection

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2005

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tom 45

|

nr 1

369-374

19

Różowokwiatowe robinie (Robinia L., Fabaceae) dziczejące w Polsce

51%

Zielinski J., Biel G., Danielewicz W., Tomaszewski D., Gawlak M.

Rocznik Polskiego Towarzystwa Dendrologicznego

|

2015

|

tom 63

20

Revision of the genus Paramacrobiotus Guidetti et al., 2009 with the description of a new species, re-descriptions and a key

51%

Kaczmarek L., Gawlak M., Bartels P. J., Nelson D. R., Roszkowska M.

Annales Zoologici

|

2017

|

tom 67

|

nr 4

Based on the original species descriptions, a review of the genus Paramacrobiotus was conducted. We divided the genus into two subgenera, Microplacoidus subgen. nov. and Paramacrobiotus subgen. nov., based on the presence or absence of a microplacoid, and characterized species within the genus based on seven different types of eggs. In a moss sample collected in Ecuador, Paramacrobiotus (Paramacrobiotus) spinosus sp. nov., was found. The new species differs from all species of the subgenus Paramacrobiotus by the presence of richtersi type eggs and from other species by morphometric characters. Additionally, in the Ecuadorian material we found P. (Microplacoidus) magdalenae comb, nov., which is the first record of this species in Ecuador, and we provide the full set of measurements for this species, not included in the original description. An additional new record is P. (M.) alekseevi comb. nov. found in Vietnam for the first time. After examining microscope slides from the Iharos’ collection deposited in the Hungarian Natural History Museum, we prepared re-descriptions of P. (P.) csotiensis comb nov., P. (M.) submorulatus comb. nov. and P. (M.) wauensis comb. nov. Based on the morphological and morphometric characters of adults and eggs, we developed a diagnostic key to the genus Paramacrobiotus.

Ograniczanie wyników

Szkolne Ostoje Przyrody

Zanieczyszczenia biologiczne jako problem bezpieczeństwa żywności

Owoc wawrzynka (Daphne L., Thymelaeaceae) - pestkowiec czy jagoda?

Wplyw nadekspresji ferrytyny w skielkowanych ziarniakach pszenicy zwyczajnej na tempo rozwoju wolka zbozowego [Sitophilus granarius L.]

Noradrenaline modulates the membrane potential in medial prefrontal cortex pyramidal neurons via β1-adrenergic receptors and HCN channels

Applicability of zymograms for detection of granary weevil (Sitophilus granarius L.) alfa-amylases left on grain after insects infestation

Gelatinolytic activity is present in the interchromatin space of the neuronal cell nucleus

Kłopotliwe derenie - Cornus alba L. i C. sericea L. (Cornaceae). Dwa gatunki czy jeden?

Morphological characters of copulatory organs and eggs of Arion rufus and Arion lusitanicus

Macrobiotus naskreckii sp. nov., a new tardigrade (Eutardigrada: Macrobiotidae) of the hufelandi group from Mozambique

The effect of copper sulphate on the alimentary tract of Arion vulgaris Moquin-Tandon, 1855

Muscarnic receptor regulation of pyramidal neuron membrane potential in the medial prefrontal cortex (mPFC)

Using scanning electron microscopy to study slug morphology

Ultrastructure of Hepatopancreas in Arion vulgaris and Arion rufus

Wpływ czasu żerowania chrząszczy wołka zbożowego (Sitophilus granarius L.) na ziarnie pszenicy na masę ziaren i zawartość wyekstrahowanego białka

Anatomical study on the developing pericarp of selected Rosa species (Rosaceae)

Outsourcing uslug IT - ekonomia, efektywnosc, nowoczesnosc...

Zastosowanie metody immunochemicznej do oznaczania skazenia ziarna zboz przez wolka zbozowego [Sitophilus granarius L.]

Różowokwiatowe robinie (Robinia L., Fabaceae) dziczejące w Polsce

Revision of the genus Paramacrobiotus Guidetti et al., 2009 with the description of a new species, re-descriptions and a key