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1
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
Content available

Stress-tolerant antagonistic rhizobacteria isolated from the medicinal plant Tinospora cordifolia

100%
Vyas P., Kaur A.
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
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2018
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tom 99
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nr 2
2

An exonic G894T variant of endothelial nitric oxide synthase gene as a risk factor for ischemic stroke in North Indians

81%
Kaur K., Uppal A., Kaur A.
Acta Neurobiologiae Experimentalis
|
2015
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tom 75
|
nr 4
Nitric oxide (NO) synthesized by endothelial nitric oxide synthase (eNOS) enzyme is a critical regulator of cerebrovascular homeostasis. Genetic variability of G894T and intronic 4ab polymorphism in eNOS could affect the expression and activity of eNOS enzyme, modulating NO levels in endothelium. This results in endothelial dysfunction, which can contribute to the pathogenesis of ischemic stroke. The purpose of the study was to evaluate the association of eNOS genetic polymorphisms (G894T and 4ab) with the occurrence of ischemic stroke through various genetic models. Both polymorphisms were genotyped in 120 ischemic stroke patients diagnosed with MRI and other ancillary techniques and 101 control subjects free of neurological abnormalities, using PCR-RFLP technique and direct PCR respectively. The genotypes of both G894T and 4ab variants were found to be in Hardy Weinberg equilibrium for cases and controls. The significant variation was observed in the genotypic and allelic frequencies for G894T polymorphism between cases and controls, indicating the association of G894T variability with ischemic stroke. However, the difference between cases and controls was insignificant for eNOS 4ab polymorphism with regard to genotypic and allelic distribution. Except for recessive model, both dominant (GT/TT vs. GG) and co-dominant (TT vs. GT or GT vs. GG) models indicated nearly two-fold and 1.93 increased risk of ischemic stroke for G894T polymorphism, but none of them suggested the influence of eNOS 4ab polymorphism on ischemic stroke susceptibility. Haplotype analysis revealed the higher frequency of GT-4bb genotype combination in cases as compared to controls, but without significant difference. The study concluded that SNP G894T variant is associated with ischemic stroke and might contribute to ischemic stroke susceptibility in North Indians. However, this outcome needs to be confirmed by studies with large sample size.
3

Biochemical and histological changes during in vitro rooting of microcuttings of Bacopa monnieri (L.) Wettst

81%
Goel A., Kaur A., Kumar A.
Acta Physiologiae Plantarum
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2018
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tom 40
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nr 03
The present study was conducted to investigate the biochemical changes vis-à-vis histological changes during adventitious rooting of microcuttings of Bacopa monnieri (L.) Wettst. The rooting in these microcuttings was induced on basal MS medium and medium supplemented with different concentrations of indole-3-acetic acid and indole-3-butyric acid (IBA). Presence of lower auxin concentration (1.0 µM) in the medium enhanced rooting and significantly improved number of roots per shoot but maximum root length was observed on basal MS medium. Histological studies were conducted to identify different phases of rooting in these microcuttings. The root meristemoids with distinct polarity become visible after 3 days and mark the beginning of in vitro root initiation phase. It was followed by primordia elongation, root emergence and visible rooting on the 5th day of culture on medium supplemented with auxins. Biochemical studies were also conducted from basal portions of microcuttings cultured on MS medium supplemented with 1.0 µM IBA and control (basal MS medium) from 0 to 7 days. Total carbohydrate content was lower during initial periods (up to day 1) and was found to increase during root initiation and primordia development, which reflects high energy demands for active cell divisions. A significantly higher level of phenols was recorded in microcuttings on medium supplemented with IBA. Polyphenol oxidase, peroxidase (POX), ascorbate peroxidase activities were also found to vary during different phases of rhizogenesis. Early phases were also marked with the lower activities of POX and IAAO. This study revealed significant role of enzymes, sugars and phenols during different phases of rooting.
4
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Stress-tolerant antagonistic plant growth-promoting rhizobacteria from Zea mays

81%
Kaur A., Devi S. R., Vyas P.
Journal of Plant Protection Research
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2018
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tom 58
|
nr 2
Biological control of plant diseases is strongly emerging as an effective alternative to the use of chemical pesticides and fungicides. Stress tolerance is an important attribute in the selection of bacteria for the development of microbial inoculants. Fourteen salt-tolerant bacteria showing different morphological features isolated from the rhizosphere of maize were evaluated for different plant growth-promoting activities. All isolates showed auxin production ranging from 5 to 24 μg ⋅ ml–1 after 48 h incubation in tryptophan supplemented media. Phosphate solubilization ranged from 15 to 419 μg ⋅ ml–1. 1-aminocycloproprane- 1-carboxylate (ACC) deaminase activity was shown by 6 isolates, ammonia production by 9 isolates, siderophore production by 8 isolates while HCN production by 4 isolates. Four bacterial isolates with all plant growth-promoting properties also showed strong antagonistic activities against Fusarium oxysporum, F. verticillioides, Curvularia lunata and Alternaria alternata and abiotic stress tolerance against salinity, temperature, pH and calcium salts. Two selected bacterial isolates significantly enhanced the growth of pea and maize test plants under greenhouse conditions. The bacterial isolate M1B2, which showed the highest growth promotion of test plants, was identified as Bacillus sp. based on phenotypic and 16S rDNA gene sequencing. The results indicated that Bacillus sp. M1B2 is a potential candidate for the development of microbial inoculants in stressful environments.
5

Comparision of seed quality at physiological maturity and harvest maturity of two pigeon pea [Cajanus cajan L. Millsp] cultivars

81%
Khattra S., Kaur A., Singh G.
Acta Agrobotanica
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1997
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tom 50
|
nr 1-2
49-52
Seed quality at physiological and harvest maturity of two pigeon pea cultivars viz. AL15 and T21 was compared. Seeds of both cultivars attained maximum dry weights, germination potential and seed vigor in terms of root/shoot lengths and dry weights of seedlings at 35 DAA in cv AL15 and 46 DAA in cv T21 denoting their physiological maturities. The levels of endogenous seed reserves like starch, proteins, lipids, DINA and RNA were comparable at physiological and harvest maturities. The seeds at physiological maturities i.e. at 35 DAA in cv AL15 and 46 DAA in cv T21 attained most of the required characters associated with the harvest of crop.
6

Kinetic behaviour of soluble invertases may be connected with the differential heat sensitivity of two wheat genotypes

80%
Asthir B., Kaur A., Basra A. S.
Acta Physiologiae Plantarum
|
1998
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tom 20
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nr 3
Soluble acid and neutral invertases purified from the midmilky stage grains of a heat-susceptible (HD 2329) and a heat-tolerant (TDR-195) genotype of wheat (Triticum aestivum L.) showed marked differences in pH optima, Km and Vmax values. Both the invertases were more thermostable in TDR-195 because of inherent heat-tolerance ability of this genotype. Inhibition of enzyme activity by iodine and its regeneration by β-mercaptoethanol implied the involvement of sulphydryl groups for enzyme action. Inhibitory effect of sulphydryl group modifiers and various metal ions was much more pronounced in the susceptible genotype than in the tolerant genotype. Overall, it appears that the observed differences in thermal stability and kinetic behaviour of invertases may be connected to the differential heat sensitivity of the two genotypes studied.
7

Cultivar variation in heat stability and kinetic properties of soluble invertase in wheat grains

80%
Asthir B., Kaur A., Basra A. S.
Acta Physiologiae Plantarum
|
1998
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tom 20
|
nr 4
Soluble invertase from mid-milky stage grains of two wheat (Triticum aestivum L.) varieties, namely Kalyansona and PBW 343 was isolated and purified by employing ammonium sulphate precipitation, gel filtration on Sephadex G-150 and DEAE-cellulose column chromatography. Invertase from Kalyansona exhibited greater heat stability (50 °C) compared to PBW 343 (35 °C). By employing photo-oxidation and chemical modification methods, and by studying the effect of pH on Km and Vmax, the involvement of histidine, sulphydryl and α-carboxyl groups in the active site of the enzyme was indicated. The enzyme was completely inhibited by HgCl₂ and DTNB. ZnSO₄, MgSO₄, KCl, CaCl₂, EDTA and pyridoxine were strong inhibitors in PBW 343 but not in Kalyansona. The two varieties also showed differential response in respect to thermodynamic properties of the enzyme, i.e. energy of activation (Ea), enthalpy change (ΔH) and entropy change (ΔS). Overall the results suggest that genetic differences exist in soluble invertase properties of wheat grains and that the thermal adaptation of the enzyme is reflected in its altered kinetic behaviour.
8
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Attitude and acceptance of Covid-19 vaccine amongst medical and dental fraternity - a questionnaire survey

61%
Kaur A., Kaur G., Kashyap A., Singh G., Singh Sandhu H., Khilji I., Singh Gambhir R.
Roczniki Państwowego Zakładu Higieny
|
2021
|
tom 72
|
nr 2
9
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Content available

Covid-19 and mucormycosis (Black Fungus): An epidemic within the pandemic

61%
Gambhir R. S., Aggarwal A., Bhardwaj A., Kaur A., Sohi R. K., Mehta S.
Roczniki Państwowego Zakładu Higieny
|
2021
|
tom 72
|
nr 3
10
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
Content available

Identifying monkeypox: do dental professionals have adequate knowledge and awareness?

61%
Kaur A., Goel R., Singh R., Bhardwaj A., Kumari R., Gambhir R. S.
Roczniki Państwowego Zakładu Higieny
|
2022
|
tom 73
|
nr 3
Background. The emergence of monkeypox has presented a new challenge for health agencies around the globe. There is significant increase in the number of cases that too in non-endemic countries with more than 18000 cases reported worldwide. Objective. The present study was conducted to assess knowledge and awareness regarding monkeypox among dental professionals. Materials and Method. The present cross-sectional study among 410 subjects who were residing in a northern state of the country. Informed consent was obtained from all the subjects. Study sample was selected on the basis of Systematic random sampling methodology. A self-designed close-ended questionnaire written in English and verified by experts was utilized for the study. The questionnaire was delivered personally to study subjects to collect the required information. Chi-square test and ANOVA were used for statistical analysis. Results. One-fourth (24.8%) of the subjects never heard about monkeypox disease. Negative response was given by 44.8% of subjects regarding resemblance of monkeypox with small pox. Only 31.2% of subjects had knowledge regarding oral manifestations of the disease. High knowledge scores were reported by only 28% of subjects. Higher knowledge levels were significantly related to education level and working profile of study subjects. Online media (Internet) was preferred as the main source to obtain more information by 42.2 of subjects. Conclusion. There was low level of knowledge regarding monkeypox among study subjects. There is an urgent need for dental professionals to keep themselves updated with recent knowledge on new emerging infectious diseases.
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