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Opracowanie eukariotycznego systemu ekspresyjnego dzialajacego na podstawie aktywnosci rekombinazy Flp

100%

Wicher K. B., Jura N., Kowanetz M., Lorenowicz M., Rafalska I., Szybalski W., Bereta M.

Biotechnologia

1999

nr 1

144-152

Pulsating electromagnetic field stimulation prevents cell death of puromycin treated U937 cell line

84%

Kaszuba-Zwoinska J., Wojcik K., Bereta M., Ziomber A., Pierzchalski P., Rokita E., Marcinkiewicz J., Zaraska W., Thor P.

Journal of Physiology and Pharmacology

2010

tom 61

nr 2

Aim of study was to verify whether pulsating electromagnetic field (PEMF) can affect cancer cells proliferation and death. U937 human lymphoid cell line at densities starting from 1x106 cells/ml to 0.0625x106 cells/ml, were exposed to a pulsating magnetic field 50Hz, 45±5 mT three times for 3 h per each stimulation with 24 h intervals. Proliferation has been studied by counting number of cells stimulated and non-stimulated by PEMF during four days of cultivation. viability of cells was analyzed by APC labeled Annexin V and 7-AAD (7-amino-actinomycin D) dye binding and flow cytometry. Growing densities of cells increase cell death in cultures of U937 cells. PEMF exposition decreased amount of cells only in higher densities. Measurement of Annexin V binding and 7-AAD dye incorporation has shown that density-induced cell death corresponds with decrease of proliferation activity. PEMF potentiated density-induced death both apoptosis and necrosis. The strongest influence of PEMF has been found for 1x106cells/ml and 0.5x106 cells/ml density. To eliminate density effect on cell death, for further studies density 0.25x106 cells/ml was chosen. Puromycin, a telomerase inhibitor, was used as a cell death inducer at concentration 100 µg/ml. Combined interaction of three doses of puromycin and three fold PEMF interaction resulted in a reduced of apoptosis by 24,7% and necrosis by 13%. PEMF protects U937 cells against puromycin- induced cell death. PEMF effects on the human lymphoid cell line depends upon cell density. Increased density induced cells death and on the other hand prevented cells death induced by puromycin.

Ograniczanie wyników

1 Biotechnologia

1 Journal of Physiology and Pharmacology

2 Bereta M.

1 Jura N.

1 Kaszuba-Zwoinska J.

1 Kowanetz M.

1 Lorenowicz M.

1 Marcinkiewicz J.

1 Pierzchalski P.

1 Rafalska I.

1 Rokita E.

1 Szybalski W.

1 Thor P.

1 Wicher K. B.

1 Wojcik K.

1 Zaraska W.

1 Ziomber A.

1 2010

1 1999

Wyniki wyszukiwania

Opracowanie eukariotycznego systemu ekspresyjnego dzialajacego na podstawie aktywnosci rekombinazy Flp

Pulsating electromagnetic field stimulation prevents cell death of puromycin treated U937 cell line