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Infekcje bakteriofagami mezofilnych szczepow bakterii z rodzaju Lactococcus w mleczarstwie

100%

Hejnowicz M. S., Bardowski J.

Przegląd Mleczarski

2005

nr 07

4-7

Biodiversity of Lactococcus lactis bacteriophages in Polish dairy environment

80%

Szczepanska A. K., Hejnowicz M. S., Kolakowski P., Bardowski J.

Acta Biochimica Polonica

2007

tom 54

nr 1

We present here the results of an exploration of the bacteriophage content of dairy wheys collected from milk plants localized in various regions of Poland. Thirty-three whey samples from 17 regions were analyzed and found to contain phages active against L. lactis strains. High phage titer in all whey samples suggested phage-induced lysis to be the main cause of fermentation failures. In total, over 220 isolated phages were examined for their restriction patterns, genome sizes, genetic groups of DNA homology, and host ranges. Based on DNA digestions the identified phages were classified into 34 distinct DNA restriction groups. Phage genome sizes were estimated at 14-35 kb. Multiplex PCR analysis established that the studied phages belong to two out of the three main lactococcal phage types - c2 and 936, while P335-type phages were not detected. Yet, analyses of bacterial starter strains revealed that the majority of them are lysogenic and carry prophages of P335-type in their chromosome. Phage geographical distribution and host range are additionally discussed.

Ograniczanie wyników

1 Acta Biochimica Polonica

1 Przegląd Mleczarski

2 Bardowski J.

2 Hejnowicz M. S.

1 Kolakowski P.

1 Szczepanska A. K.

1 2007

1 2005

Wyniki wyszukiwania

Infekcje bakteriofagami mezofilnych szczepow bakterii z rodzaju Lactococcus w mleczarstwie

Biodiversity of Lactococcus lactis bacteriophages in Polish dairy environment