Wyniki wyszukiwania

1

Alpha-galactosidase in immobilized cells of Citrullus vulgaris L.

100%

Stano J., Micieta K., Tokhtaeva E., Fulmekova M.

Herba Polonica

|

2005

|

tom 51

|

nr 3-4

Cells of suspension culture Citrullus vulgaris cv. "Samara" were permeabilized by Tween 80 and immobilized by glutaraldehyde. The highest a-galactosidase activity was at pH 5.4 and 60°C. The hydrolysis of substrate was linear for 3.5 h reaching 65-70% conversion of the substrate. The cells characterized with high enzyme activity, and stability in long-term storage showed convenient physico-mechanical properties (physical protection from shear forces and easy separation of product from biocatalysts).

2

Presentation of beta-galactosidase activity secreted by plant cells

100%

Stano J., Micieta K., Grancai D., Blanarikova V.

Herba Polonica

|

2007

|

tom 53

|

nr 1

Extracellular plant β-galactosidase from opium poppy and celandine was detected on agar plates by the presence of dyed zones from 1-naphthyl-β-D-galactopyranoside used as a synthetic substrate. Evaluating the azo-dye zones assessed the degree β-galactosidase activity. No coloration of the agar medium was observed on non-inoculated parts, in medium inoculated with heat – inactivated cells (10 min. at 100oC) or in medium without substrate with all calli tested. On the agar plates with substrate and sterile opium poppy, celandine or gherkin seedlings (2–6 days old) changes in coloration were observed showing the release of β-galactosidase from the roots during germination.

3

Saccharase in free and immobilized cells of Papaver somniferum L.

88%

Stano J., Micieta K., Kovacs P., Neubert K., Menon V. P.

Herba Polonica

|

2003

|

tom 49

|

nr 1-2

Cell suspension of Papaver somniferum L. (opium poppy) was permeabilized by Tween 80 and immobilized by glutaraldehyde. The highest saccharase activity was at pH 4.6 and 50°C. The hydrolysis of the substrate was linear for 5 h reaching 69% of conversion, A very good storage stability was achieved when using dry catalyst, or a solution of 0.15 M NaCl with the addition of chloramphenicol, (l-methyldodecyl)-dimethylamin-4-oxide (ATDNO), Chlortetracycline hydrochloride (CLCTC) or by freezing the immobilized cells in 0.15 M NaCl. The cells characterized by high enzyme activity and stability in long-term storage showed convenient technological and physicomechanical properties.

4

Identification and determination of extracellular saccharase in cell suspension of Chelidonium majus L.

76%

Micieta K., Stano J., Korenova M., Blanarikova V., Fulmekova M., Matejka P.

Herba Polonica

|

2005

|

tom 51

|

nr 1-2

A simple and rapid procedure for identification and determination of extracellular saccha- rase is described, using a culture medium of Chelidonium majus L. (celandine) cell suspension cultures. Sucrose was used as a substrate for determination of the extracellular and intracellular activities of the studied enzyme. The culture medium (without cells) was used for identification and determination of extracellular enzyme activity. Intracellular activity was estimated using cell suspension.

5

Beta-galactosidase in immobilized cells of gherkin Cucumis sativus L.

64%

Stano J., Nemec P., Bezakova L., Kakoniova D., Kovacs P., Neubert K., Liskova D., Andriamainty F., Micieta K.

Acta Biochimica Polonica

|

1998

|

tom 45

|

nr 2

Cell suspensions of gherkin (Cucumis sativus L.) were permeabilized by Tween-80, and immobilized by glutaraldehyde. β-Galactosidase showed pH optimum at 4.9 and temperature optimum at 58 °C. The enzyme catalysed hydrolysis was linear for 3 h with 60-68% conversion of the substrate. The cells characterized by high β-galactosidase activity and stability on long-term storage showed valuable technological properties.

Ograniczanie wyników

4 Herba Polonica

1 Acta Biochimica Polonica

5 Micieta K.

5 Stano J.

2 Blanarikova V.

2 Fulmekova M.

2 Kovacs P.

2 Neubert K.

1 Andriamainty F.

1 Bezakova L.

1 Grancai D.

1 Kakoniova D.

1 Korenova M.

1 Liskova D.

1 Matejka P.

1 Menon V. P.

1 Nemec P.

1 Tokhtaeva E.

1 2007

2 2005

1 2003

1 1998

Alpha-galactosidase in immobilized cells of Citrullus vulgaris L.

Presentation of beta-galactosidase activity secreted by plant cells

Saccharase in free and immobilized cells of Papaver somniferum L.

Identification and determination of extracellular saccharase in cell suspension of Chelidonium majus L.

Beta-galactosidase in immobilized cells of gherkin Cucumis sativus L.