As part of an inventory of aquatic invertebrates, our work involved species sampled from an archaeological site protected by the Algerian Ministry of Culture. Samples were taken monthly during the five-month, rainy period (February 2019-June 2019), in five separate vestiges (stations) selected according to the presence of water (rainwater). Systematic identification was carried out on 14,890 individuals sampled, identifying 18 species belonging to 11 families and 4 classes: Insecta, Arachnidia, Chilopoda, Crustacae. And the last one was the most representative in terms of abundance and taxonomic richness. On the other hand, a physicochemical analysis of ten parameters (temperature, hydrogen potential, electrical conductivity, total hardness, alkalimetric titer, complete alkalimetric titer, chemical oxygen demand, chlorides, calcium and magnesium) was carried out to determine the distribution of species. Analysis of the data obtained showed that electrical conductivity seems to be the most influential factor in the distribution of the majority of species among the five studied stations.