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Promotive effect of 5-aminolevulinic acid on chlorophyll, antioxidative enzymes and photosynthesis of Pakchoi (Brassica campestris ssp. chinensis var. communis Tsen et Lee)

100%

Memon S. A., Hou X., Wang L., Li Y.

Acta Physiologiae Plantarum

2009

tom 31

nr 1

The objective of this article is to study the effect of 5-aminolevulinic acid (ALA) and enhanced chlorophyll content, antioxidative enzymes and photosynthesis rate by foliar application of ALA. We evaluated three concentrations (control-distilled water, T1-50 mg l⁻¹, T2-150 mg l⁻¹, T3-250 mg l⁻¹) of ALA and seven cultivars, ‘‘Sanchidaye’’ (Sa-1), ‘‘Lichuandasuomian’’ (Li-1), ‘‘Aijiaohuang’’ (Ai-1), ‘‘Qingyou’’ No. 4 (Qi-1), ‘‘Aikang’’ No. 5 (Ak-1), ‘‘Hanxiao’’ (Ha-1) and ‘‘Shulv’’ (Sl⁻¹). ‘‘Ak-1’’ showed strongest response of POD (peroxidase) enzyme activity (0.4 U g⁻¹ min⁻¹) in 250 mg l⁻¹ ALA solution. The highest CAT (catalase) activity (0.8 U g⁻¹ min⁻¹) after administration of 250 mg l⁻¹ ALA was observed in ‘‘Li-1’’. Meanwhile, highest (1.42 mg l⁻¹) total chlorophyll content was also observed in ‘‘Ak-1’’, when leaves were treated in 50 mg l⁻¹ ALA, ‘‘Li-1’’ and ‘‘Ai-1’’ showed strongest response of specific activity of superoxide dismutase (SOD) in 50 mg l⁻¹ and 50 mg l⁻¹ ALA. Two hundred and fifty milligram per milliliter of ALA-treatment significantly improved the net photosynthetic rate.

High-frequency adventitious shoots regeneration from leaf of non-heading Chinese cabbage (Brassica campestris ssp. chinensis) cultured in vitro

100%

Memon S. A., Hou X., Zhu B., Wolukau J. N.

Acta Physiologiae Plantarum

2009

tom 31

nr 6

The objective of the present work was selection of cultivar and suitable medium for regenerating shoots from leaf segments of non-heading Chinese cabbage. We evaluated six types of supplemented media with 2.0, 5.0 and 10.0 mg l⁻¹ 6-BA; 1.0 and 2.0 mg l⁻¹ TDZ; 0.1, 0.3, 0.5, 0.8 and 1.0 mg l⁻¹NAA; 3.0, 5.0 and 7.5 mg l⁻¹AgNO₃; 0.01 mg l⁻¹ 2–4, D and 4.0 mg l⁻¹ KT for shoot regeneration and six cultivars ‘‘Sanchidaye’’, ‘‘Liuchuandasuomian’’, ‘Qingyou 4’’, ‘‘Liangbaiye’’, ‘‘AiKang 5’’ and ‘‘Hanxiao F3’’, furthermore for root formation three types of supplemented media with 0.2, 0.3, 0.5 mg l⁻¹ NAA, and for survival rate two types of base media: turf + vermiculite + manure (1:2:0.2) and soil + vermiculite (1:2). Culturing leaf segments on MS medium supplemented with 2 mg l⁻¹ TDZ; 0.5 mg l⁻¹ NAA and 7.5 mg l⁻¹ AgNO₃ gave the highest number of shoots per leaf segment (66) while roots were best formed on the medium supplemented with 0.2 mg l⁻¹ NAA. Survival rate was highest (61.6%) in the turf: vermiculite: manure (1:2:0.2) medium. The highest percentage of responding leaf segments, number of shoots per leaf segment, rooting percentage and survival rate were observed in ‘‘Liuchuandasuomian’’. The plantlets were transferred to the soil and grown into mature plants in pots. These results could be used for preliminary selections of cultivars to transfer disease resistance (Bt) gene through agrobacterium in non-heading Chinese cabbage.

Ograniczanie wyników

2 Acta Physiologiae Plantarum

2 Hou X.

2 Memon S. A.

1 Li Y.

1 Wang L.

1 Wolukau J. N.

1 Zhu B.

2 2009

Wyniki wyszukiwania

Promotive effect of 5-aminolevulinic acid on chlorophyll, antioxidative enzymes and photosynthesis of Pakchoi (Brassica campestris ssp. chinensis var. communis Tsen et Lee)

High-frequency adventitious shoots regeneration from leaf of non-heading Chinese cabbage (Brassica campestris ssp. chinensis) cultured in vitro