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1

Identification and partial characterization of two cysteine proteases from castor bean leaves (Ricinus communis L.) activated by wounding and methyl jasmonate stress

100%
Maciel F. M., Salles M. C., Retamal C. A., Gomes V. M., Machado L. T.
Acta Physiologiae Plantarum
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2011
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tom 33
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nr 5
Jasmonates are signaling molecules that play key roles in wound response and regulate the biosynthesis of many defensive proteins, including proteases. In this study, we investigate the effects of wounding and methyl jasmonate (MJ) application on the protein expression pattern of Ricinus communis L. leaves and on proteolytic activity. Gelatin zymography demonstrated that both MJ and mechanical wounding induce alterations in the proteolytic pattern of castor bean leaves (R. communis L.). Expression of two cysteine proteases (38 and 29 kDa) was induced by the treatments employed; however, MJ induced a higher protease level than mechanical wounding during the stress period (24, 48, and 72 h). The increase in protease activity mirrors the decline in soluble protein content and rubisco degradation that may indicate initiation of senescence in castor plants. The 29 kDa protease has an acidic optimal pH; whereas the 38 kDa protease has a neutral optimum activity. Both proteases were almost completely inhibited by E-64 and cystatin. The significant induction of these proteins by MJ suggests a possible role of cysteine proteases in leaf senescence as well as their involvement in regulating both the wound response and MJ in castor bean plants.
2

Partial characterization and antimicrobial activity of peptides from Amburana cearensis seeds against phytopathogenic fungi and yeasts

86%
dos Santos P. H. A., dos Santos I. S., Melo V. M. M., Vasconcelos I. M., Carvalho A. d. O., Gomes V. M.
Acta Physiologiae Plantarum
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2010
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tom 32
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nr 3
The aim of this work was to study the antifungal action of a protein and peptide-rich fraction from Amburana cearensis seeds. Initially, proteins were extracted from seed flour in phosphate buffer and re-extracted with 1 M lithium chloride. The products obtained from both extractions were precipitated with ammonium sulfate at 70% saturation, and the precipitates were re-suspended in distilled water, heated at 80℃ for 15 min and clarified by centrifugation at 12,000×g. The obtained fractions from both extractions were dialyzed, recovered by lyophilization and visualized in Tris–Tricine/SDS gel electrophoresis. The fractions obtained were rich in proteins of low molecular weight and were submitted to antifungal assays. Fraction S4 (extraction with phosphate buffer) inhibited the fungi, Colletotrichum lindemuthianum, Fusarium oxysporum, Fusarium solani, Candida albicans and Saccharomyces cerevisiae. Fractions P2 (re-extraction with lithium chloride) had a low or non-inhibitory effect on the fungi tested.
3

Characterization of Capsicum annuum L. leaf and root antimicrobial peptides: antimicrobial activity against phytopathogenic microorganisms

58%
Pereira L. S., do Nascimento V. V., Fereira Ribeiro S. F., Rodrigues R., Fernandes K. V. S., Carvalho A. O., Vasconcelos I. M., Bento C. S., Sudre C. P., Zottich U., Gomes V. M.
Acta Physiologiae Plantarum
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2018
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tom 40
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nr 06
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