Water stress as the one of the important limiting factors in plant growth was applied with polyethylene glycol (PEG) 6000 in solutions of 10, 20, 30 and 40% strengths that achieve water deficit levels of -0.15, -0.49, -1.03 and -1.76 MPa, respectively. After 24 hours treatment, the roots and leaves respirations of two maize (Zea mays L.) cultivars – 704 and 301 – were determined in various concentrations of PEG 6000 in light and dark conditions. Respiration rate declined in leaves and roots with increasing PEG concentrations. Decreases of oxygen uptake in roots and leaves of 704 variety were significantly (p < 0.01) higher than 301 variety and in light conditions were significantly (p < 0.01) higher than in dark. The rate of respiration in the light was lower than in darkness and the decrease of oxygen uptake in water stress in dark conditions was lower than in light.