Short-term storage of semen in cooling conditions (+4℃) is one way to perfect artificial fish reproduction. In this experiment, we attempted to add antioxidants (vitamins C and E, glutathione and cysteine) during the storage of Arctic char (Salvelinus alpinus) and rainbow trout (Oncorhynchus mykiss) semen. An analysis of the CASA parameters showed that addition of the antioxidants to semen during storage did not benefit spermatozoa motility of studied salmonids. An analysis of the parameters showed that added vitamins C and E did not influence the sperm motility of Salmonidae during semen storage. The addition of glutathione and cysteine significantly worsened the vitality of Arctic char and rainbow trout sperm.
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