Wyniki wyszukiwania

1

Zaplodnienie wspomagane u koni

100%

Kochan J., Tischner M.

Medycyna Weterynaryjna

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2006

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tom 62

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nr 03

262-265

Problems associated with in vitro fertilization of horses were the subject of the present review. The paper presents the intracytoplasmic sperm injection (ICSI) technique. Oocytes for in vitro fertilization can be obtained either from living or slaughtered mares and then cultured. Afterwards they are washed and only those containing 1 polar body are used for microinjection. All kinds of spermatozoa (heads, immobile spermatozoa, dead and abnormal) can be used. Several foals have been born in the USA, Europe and Australia after in vitro fertilization. The technique can be successfully applied in breeding programs which accept stallions with low volume or motility spermatozoa.

2

Zapłodnienie wspomagane oraz klonowanie koni

84%

Galli C., Cololeoni S., Lagutina I., Duchi R., Lazzari G.

Prace Komisji Nauk Rolniczych, Leśnych i Weterynaryjnych. Polska Akademia Umiejętności

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2014

|

tom 20

3

Próba uzyskania zarodków końskich po zapłodnieniu wspomaganym (ICSI)

84%

Kochan J., Tischner M., Nowak A., Baranska M.

Medycyna Weterynaryjna

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2012

|

tom 68

|

nr 01

The aim of this study was to obtain embryos after assisted fertilization of oocytes collected from slaughtered mares and matured in vitro by an intracytoplasmic sperm injection (ICSI) and to establish optimal conditions for the in vitro culture of the embryos. Oocytes were collected by the scraping method and cultured in vitro in TCM with Earl’s salts supplemented with 20% FBS, 5 g/ml FSH, estradiol 1 g/ml, pyruvate and glutamine. After a 30-hour in vitro culture oocytes that had reached the metaphase II stage were subjected to an intracytoplasmic sperm microinjection (ICSI) with frozen semen. After thawing, a motile sperm selection was carried out by the „swim up” method. Then the sperm was immobilized, aspirated into an injection pipette, and introduced into the cytoplasm of an oocyte. Microinjected oocytes were transferred onto a culture medium: DMEM F12 or SOF. The control group consisted of oocytes subjected to microinjection without sperm (sham ICSI). Oocytes that did not show signs of development and embryos in different developmental stages were fixed and stained with Hoechst fluorochrome. The in vitro culture of embryos in the DMEM F12 medium produced only early stages of development (pronucleus, two-blastomere embryos). In contrast, the in vitro culture in the SOF medium produced embryos in the morula stage (6.8%). In the control group, no signs of embryonic development were observed.

4

Aktualne osiągnięcia naukowe i praktyczne w rozrodzie koni

67%

Kosiniak-Kamysz K., Okolski A.

Postępy Nauk Rolniczych

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2007

|

tom 59

|

nr 3

5

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Biotechnologia w rozrodzie koni

67%

Tischner M.

Życie Weterynaryjne

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2006

|

tom 81

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nr 01

19-31

6

Zapłodnienie i zapłodnienie in vitro. Nagroda Nobla z biologii lub medycyny 2010

59%

Maleszewski M.

Kosmos

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2011

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tom 60

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nr 1-2

Ograniczanie wyników

2 Medycyna Weterynaryjna

1 Kosmos

1 Postępy Nauk Rolniczych

1 Prace Komisji Nauk Rolniczych, Leśnych i Weterynaryjnych. Polska Akademia Umiejętności

1 Życie Weterynaryjne

3 Tischner M.

2 Kochan J.

1 Baranska M.

1 Cololeoni S.

1 Duchi R.

1 Galli C.

1 Kosiniak-Kamysz K.

1 Lagutina I.

1 Lazzari G.

1 Maleszewski M.

1 Nowak A.

1 Okolski A.

1 2014

1 2012

1 2011