Wyniki wyszukiwania

Wyszukiwano:
w słowach kluczowych: zakazna martwica trzustki

Sortuj według:

Ogranicz wyniki do:

Izolacja wirusow posocznicy krwotocznej i zakaznej martwicy trzustki ryb lososiowatych w Polsce

100%

Antychowicz J., Wejman M., Grawinski E.

Medycyna Weterynaryjna

2000

tom 56

nr 04

255-258

For the first time in Poland viral examination of rainbow trout by applying cells lines (BF-2 and EPC) and ELISA tests approved in European Community Countries have shown the presence of VHS and IPN viruses existing in the country. VHS virus was isolated at 11 farms and IPN virus at 31 farms situated in northern Poland. VHS virus was isolated both in sick fish as well as in carriers and IPN virus in carriers only. The presence of VHS and IPN viruses did not appear in trout from farms in southern Poland. The experimental infection of rainbow trout demonstrated the high virulence of one of the VHS isolates. It was found that ELISA tests for VHS and IPN are very useful in detecting viruses in tissue supernatants of fish with clinical symptoms but are not as useful in detecting viruses present in cases of low titters. The results of this investigation showed that it is necessary to intensify the monitoring of the VHSV and IPNV presence in carriers and especially in fish planned to be transferred from the north to south of Poland. This should be done by using fish cell lines and immunological methods recommended in EU countries e.g. ELISA tests. The mutual co-operation of fish breeders, veterinary officers, regional fish disease laboratories and the Fish Disease Laboratory of the National Veterinary Research Institute is imperative for the effective control of fish viral diseases.

Charakterystyka wirusa zakaźnej martwicy trzustki ryb łososiowatych i jego identyfikacja

88%

Maj-Paluch J., Reichert M.

Medycyna Weterynaryjna

2016

tom 72

nr 04

The IPN virus belongs to the Birnaviridae family, Aquabirnavirus genus. It is an enveloped, two-segment, double-stranded RNA virus. Segment A comprises two open reading frames. ORF 1 encodes VP5 protein, whereas ORF 2 encodes VP2, NS and VP3 proteins. Segment B comprises one open reading frame, which encodes VP1 protein. The VP2 gene is responsible for the virulence of the virus. The Aquabirnavirus genus is divided into two serogroups: A and B. There are 9 serotypes in serogroup A, including serotypes occurring in Europe. Serogroup B consists of one serotype. The virus grows in several cell lines and often occurs in dual infections, inhibiting the replication of other viruses. Diagnosis is based on the isolation of the virus in cell lines, serological methods and, above all, techniques of molecular biology.

Ograniczanie wyników

2 Medycyna Weterynaryjna

1 Antychowicz J.

1 Grawinski E.

1 Maj-Paluch J.

1 Reichert M.

1 Wejman M.

1 2016

1 2000

Wyniki wyszukiwania

Izolacja wirusow posocznicy krwotocznej i zakaznej martwicy trzustki ryb lososiowatych w Polsce

Charakterystyka wirusa zakaźnej martwicy trzustki ryb łososiowatych i jego identyfikacja